naa a22 4500 510759432 CHVBK 20180411083133.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s13225-013-0227-8 doi (NATIONALLICENCE)springer-10.1007/s13225-013-0227-8 An antimicrobial peptide from endophytic Fusarium tricinctum of Rhododendron tomentosum Harmaja [Elektronische Daten] [M. Tejesvi, D. Segura, K. Schnorr, D. Sandvang, S. Mattila, P. Olsen, S. Neve, T. Kruse, H. Kristensen, A. Pirttilä] Endophytic microbes have attracted considerable attention as a completely new source of pharmaceuticals in recent years. An endophytic Fusarium tricinctum was isolated from Rhododendron tomentosum, a shrub that is found widely across the northern hemisphere. The endophytic F. tricinctum produced antimicrobial compounds that were active against Staphylococcus carnosus, Candida albicans and C. utilis. The transcriptome of the F. tricinctum was sequenced, and a total of 12,006 contigs were assembled, having an N50 value of 1390bp. Analysis of the transcriptomic PD library of F. tricinctum yielded an antimicrobial peptide named Trtesin. The expression of Trtesin transcripts was >1000 fold in the mRNA library originating from PDB-grown fungi exhibiting high antimicrobial activity compared to FG4-grown fungi with no antimicrobial activity. Trtesin was cloned, expressed, and purified in pET32(a), and it consisted of 52 amino acids with 6 cysteine molecules. The molecular weight of Trtesin is 6138.92Da. An additional N-terminal sequencing was done to confirm the intact peptide, as well as to check the correct amino acid sequence. The MIC of Trtesin was determined against several bacteria as 64μg/mL, and peptide showed a mild activity against F. oxysporum in agar diffusion assay, as a zone of inhibition of 10mm was formed at 100μg of peptide. Mushroom Research Foundation, 2013 Fungal defensin nationallicence Antimicrobial nationallicence Endophyte nationallicence Transcriptome nationallicence Tejesvi M. Departments of Biology, University of Oulu, Linnanmaa, P.O. Box 3000, FIN-90014, Oulu, Finland aut Segura D. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut Schnorr K. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut Sandvang D. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut Mattila S. Departments of Chemistry, University of Oulu, Linnanmaa, P.O. Box 3000, FIN-90014, Oulu, Finland aut Olsen P. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut Neve S. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut Kruse T. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut Kristensen H. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut Pirttilä A. Departments of Biology, University of Oulu, Linnanmaa, P.O. Box 3000, FIN-90014, Oulu, Finland aut Fungal Diversity Springer Netherlands 60/1(2013-05-01), 153-159 1560-2745 60:1<153 2013 60 13225 https://doi.org/10.1007/s13225-013-0227-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13225-013-0227-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tejesvi M. Departments of Biology, University of Oulu, Linnanmaa, P.O. Box 3000, FIN-90014, Oulu, Finland aut NATIONALLICENCE 700 1- Segura D. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut NATIONALLICENCE 700 1- Schnorr K. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut NATIONALLICENCE 700 1- Sandvang D. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut NATIONALLICENCE 700 1- Mattila S. Departments of Chemistry, University of Oulu, Linnanmaa, P.O. Box 3000, FIN-90014, Oulu, Finland aut NATIONALLICENCE 700 1- Olsen P. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut NATIONALLICENCE 700 1- Neve S. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut NATIONALLICENCE 700 1- Kruse T. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut NATIONALLICENCE 700 1- Kristensen H. Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark aut NATIONALLICENCE 700 1- Pirttilä A. Departments of Biology, University of Oulu, Linnanmaa, P.O. Box 3000, FIN-90014, Oulu, Finland aut NATIONALLICENCE 773 0- Fungal Diversity Springer Netherlands 60/1(2013-05-01), 153-159 1560-2745 60:1<153 2013 60 13225 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer