naa a22 4500 510759483 CHVBK 20180411083134.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s13225-012-0216-3 doi (NATIONALLICENCE)springer-10.1007/s13225-012-0216-3 Endophytic fungi harbored in Cannabis sativa L.: diversity and potential as biocontrol agents against host plant-specific phytopathogens [Elektronische Daten] [Parijat Kusari, Souvik Kusari, Michael Spiteller, Oliver Kayser] The objective of the present work was isolation, phylogenetic characterization, and assessment of biocontrol potential of endophytic fungi harbored in various tissues (leaves, twigs, and apical and lateral buds) of the medicinal plant, Cannabis sativa L. A total of 30 different fungal endophytes were isolated from all the plant tissues which were authenticated by molecular identification based on rDNA ITS sequence analysis (ITS1, 5.8S and ITS2 regions). The Menhinick's index revealed that the buds were immensely rich in fungal species, and Camargo's index showed the highest tissue-specific fungal dominance for the twigs. The most dominant species was Penicillium copticola that could be isolated from the twigs, leaves, and apical and lateral buds. A detailed calculation of Fisher's log series index, Shannon diversity index, Simpson's index, Simpson's diversity index, and Margalef's richness revealed moderate overall biodiversity of C. sativa endophytes distributed among its tissues. The fungal endophytes were challenged by two host phytopathogens, Botrytis cinerea and Trichothecium roseum, devising a dual culture antagonistic assay on five different media. We observed 11 distinct types of pathogen inhibition encompassing a variable degree of antagonism (%) on changing the media. This revealed the potential chemodiversity of the isolated fungal endophytes not only as promising resources of biocontrol agents against the known and emerging phytopathogens of Cannabis plants, but also as sustainable resources of biologically active and defensive secondary metabolites. Mushroom Research Foundation, 2012 Cannabis sativa nationallicence Endophytic fungi nationallicence Fungal diversity nationallicence Antagonism nationallicence Botrytis cinerea nationallicence Trichothecium roseum nationallicence Kusari Parijat Department of Biochemical and Chemical Engineering, Chair of Technical Biochemistry, TU Dortmund, Emil-Figge-Str. 66, 44227, Dortmund, Germany aut Kusari Souvik Institute of Environmental Research (INFU) of the Faculty of Chemistry, Chair of Environmental Chemistry and Analytical Chemistry, TU Dortmund, Otto-Hahn-Str. 6, 44221, Dortmund, Germany aut Spiteller Michael Institute of Environmental Research (INFU) of the Faculty of Chemistry, Chair of Environmental Chemistry and Analytical Chemistry, TU Dortmund, Otto-Hahn-Str. 6, 44221, Dortmund, Germany aut Kayser Oliver Department of Biochemical and Chemical Engineering, Chair of Technical Biochemistry, TU Dortmund, Emil-Figge-Str. 66, 44227, Dortmund, Germany aut Fungal Diversity Springer Netherlands 60/1(2013-05-01), 137-151 1560-2745 60:1<137 2013 60 13225 https://doi.org/10.1007/s13225-012-0216-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13225-012-0216-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kusari Parijat Department of Biochemical and Chemical Engineering, Chair of Technical Biochemistry, TU Dortmund, Emil-Figge-Str. 66, 44227, Dortmund, Germany aut NATIONALLICENCE 700 1- Kusari Souvik Institute of Environmental Research (INFU) of the Faculty of Chemistry, Chair of Environmental Chemistry and Analytical Chemistry, TU Dortmund, Otto-Hahn-Str. 6, 44221, Dortmund, Germany aut NATIONALLICENCE 700 1- Spiteller Michael Institute of Environmental Research (INFU) of the Faculty of Chemistry, Chair of Environmental Chemistry and Analytical Chemistry, TU Dortmund, Otto-Hahn-Str. 6, 44221, Dortmund, Germany aut NATIONALLICENCE 700 1- Kayser Oliver Department of Biochemical and Chemical Engineering, Chair of Technical Biochemistry, TU Dortmund, Emil-Figge-Str. 66, 44227, Dortmund, Germany aut NATIONALLICENCE 773 0- Fungal Diversity Springer Netherlands 60/1(2013-05-01), 137-151 1560-2745 60:1<137 2013 60 13225 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer