naa a22 4500 510759556 CHVBK 20180411083134.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s13225-013-0238-5 doi (NATIONALLICENCE)springer-10.1007/s13225-013-0238-5 A new endophytic insect-associated Daldinia species, recognised from a comparison of secondary metabolite profiles and molecular phylogeny [Elektronische Daten] [Sylvie Pažoutová, Stefanie Follert, Jens Bitzer, Matthias Keck, Frank Surup, Petr Šrůtka, Jaroslav Holuša, Marc Stadler] Xylariaceous endophytes are commonly encountered in plants, and it has recently been found out that insect vectors play a major role in their life cycle, as well as in the apparent host-specificity of the fungal stromata. In this study, a new insect-associated, endophytic anamorph species of Daldinia is recognised from a comparison of a) morphological and ecological data; b) molecular phylogenetic data based on rDNA, alpha-actin and beta-tubulin genes; and c) secondary metabolite profiles based on high performance liquid chromatography/diode array/mass spectrometry (HPLC-DAD/MS) and gas chromatography/mass spectrometry (GC-MS) in comparison with authentic strains of Daldinia and other Xylariaceae. The new species, Daldinia hawksworthii, is a symbiont of the willow woodwasp, Xiphydria prolongata, which is in turn preferentially associated with Salix. A comparison of HPLC-DAD/MS profiles from standardised cultures revealed the presence of a specific compound, which was not encountered in any of the ca. 250 Daldinia strains studied for comparison. This compound was isolated after fermentation of the fungus in 10l scale by preparative chromatography. Structure elucidation by NMR spectroscopy revealed a novel pyrone, for which the name dalsymbiopyrone is proposed. The compound showed weak antimicrobial and cytotoxic effects and might be a signal molecule involved in the trilateral ecological interaction between the host plant, the insect, and its fungal symbiont. Mushroom Research Foundation, 2013 Fungi nationallicence Endophytes nationallicence Evolution nationallicence Xylariales nationallicence Bioactive metabolites nationallicence Natural products nationallicence Alpha-pyrones nationallicence Pažoutová Sylvie Institute of Microbiology of the ASCR, v.v.i., Vídeňská 1083, 14220, Prague 4, Czech Republic aut Follert Stefanie Department Microbial Drugs, Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124, Braunschweig, Germany aut Bitzer Jens IMD Natural Solutions GmbH, Otto-Hahn-Strasse 15, 44227, Dortmund, Germany aut Keck Matthias Department Microbial Drugs, Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124, Braunschweig, Germany aut Surup Frank Department Microbial Drugs, Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124, Braunschweig, Germany aut Šrůtka Petr Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 129, 165 21, Prague 6, Czech Republic aut Holuša Jaroslav Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 129, 165 21, Prague 6, Czech Republic aut Stadler Marc Department Microbial Drugs, Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124, Braunschweig, Germany aut Fungal Diversity Springer Netherlands 60/1(2013-05-01), 107-123 1560-2745 60:1<107 2013 60 13225 https://doi.org/10.1007/s13225-013-0238-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13225-013-0238-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pažoutová Sylvie Institute of Microbiology of the ASCR, v.v.i., Vídeňská 1083, 14220, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Follert Stefanie Department Microbial Drugs, Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124, Braunschweig, Germany aut NATIONALLICENCE 700 1- Bitzer Jens IMD Natural Solutions GmbH, Otto-Hahn-Strasse 15, 44227, Dortmund, Germany aut NATIONALLICENCE 700 1- Keck Matthias Department Microbial Drugs, Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124, Braunschweig, Germany aut NATIONALLICENCE 700 1- Surup Frank Department Microbial Drugs, Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124, Braunschweig, Germany aut NATIONALLICENCE 700 1- Šrůtka Petr Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 129, 165 21, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Holuša Jaroslav Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 129, 165 21, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Stadler Marc Department Microbial Drugs, Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124, Braunschweig, Germany aut NATIONALLICENCE 773 0- Fungal Diversity Springer Netherlands 60/1(2013-05-01), 107-123 1560-2745 60:1<107 2013 60 13225 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer