naa a22 4500 510760120 CHVBK 20180411083136.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11420-012-9319-3 doi (NATIONALLICENCE)springer-10.1007/s11420-012-9319-3 Lumbar Spine Intervertebral Disc Gene Delivery: A Pilot Study in Lewis Rats [Elektronische Daten] [Sheela Damle, Bernard Rawlins, Oheneba Boachie-Adjei, Ronald Crystal, Chisa Hidaka, Matthew Cunningham] Background: Basic research toward understanding and treating disc pathology in the spine has utilized numerous animal models, with delivery of small molecules, purified factors, and genes of interest. To date, gene delivery to the rat lumbar spine has only been described utilizing genetically programmed cells in a matrix which has required partial disc excision, and expected limitation of treatment diffusion into the disc. Purpose: This study was designed to develop and describe a surgical technique for lumbar spine exposure and disc space preparation, and use of a matrix-free method for gene delivery. Methods: Naïve or genetically programmed isogeneic bone marrow stromal cells were surgically delivered to adolescent male Lewis rat lumbar discs, and utilizing quantitative biochemical and qualitative immunohistological assessments, the implanted cells were detected 3days post-procedure. Results: Statistically significant differences were noted for recovery of the β-galactosidase marker gene comparing delivery of naïve or labeled cells (105 cells per disc) from the site of implantation, and between delivery of 105 or 106 labeled cells per disc at the site of implantation and the adjacent vertebral body. Immunohistology confirmed that the β-galactosidase marker was detected in the adjacent vertebra bone in the zone of surgical implantation. Conclusions: The model requires further testing in larger cohorts and with biologically active genes of interest, but the observations from the pilot experiments are very encouraging that this will be a useful comparative model for basic spine research involving gene or cell delivery, or other locally delivered therapies to the intervertebral disc or adjacent vertebral bodies in rats. Hospital for Special Surgery, 2013 gene delivery nationallicence lumbar nationallicence disc nationallicence matrix-free nationallicence Damle Sheela Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut Rawlins Bernard Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut Boachie-Adjei Oheneba Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut Crystal Ronald Weill Cornell Medical College, 10065, New York, NY, USA aut Hidaka Chisa Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut Cunningham Matthew Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut HSS Journal ® Springer-Verlag 9/1(2013-02-01), 36-41 1556-3316 9:1<36 2013 9 11420 https://doi.org/10.1007/s11420-012-9319-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11420-012-9319-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Damle Sheela Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Rawlins Bernard Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Boachie-Adjei Oheneba Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Crystal Ronald Weill Cornell Medical College, 10065, New York, NY, USA aut NATIONALLICENCE 700 1- Hidaka Chisa Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut NATIONALLICENCE 700 1- Cunningham Matthew Hospital for Special Surgery, 535 East 70th Street, 10021, New York, NY, USA aut NATIONALLICENCE 773 0- HSS Journal ® Springer-Verlag 9/1(2013-02-01), 36-41 1556-3316 9:1<36 2013 9 11420 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer