naa a22 4500 510763170 CHVBK 20180411083147.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12094-012-0889-9 doi (NATIONALLICENCE)springer-10.1007/s12094-012-0889-9 EGFR expression is linked to osteopontin and Nf-κB signaling in clear cell renal cell carcinoma [Elektronische Daten] [K. Matušan-Ilijaš, G. Damante, D. Fabbro, G. Đorđević, I. Hadžisejdić, M. Grahovac, M. Avirović, B. Grahovac, N. Jonjić, K. Lučin] Aim and background: Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase involved in many important aspects of cell biology that are related to tumorigenesis. There are opposite evidences of the role of EGFR in renal cancer and the outcome of EGFR-targeted therapies, suggesting the complexity of EGFR signaling pathways. In vitro, osteopontin (OPN) and nuclear factor kappa B (NF-κB) are thought to be involved in specific ligand-independent EGFR activation that could have a role in resistance to EGFR mAb therapy. Aim of this study was to analyze the relationship between EGFR and OPN at the protein and mRNA level, as well as their relation to NF-κB in clear cell renal cell carcinoma (CCRCC). Materials and methods: Expression of EGFR, OPN, and p65 NF-κB protein was analyzed using immunohistochemistry and compared mutually in 88 CCRCC samples. Expression of EGFR and OPN mRNAs was analyzed using quantitative Real-time PCR in 22 CCRCC samples and compared mutually and with NF-κB protein expression. Results: Epidermal growth factor receptor mRNA level was higher in CCRCC samples in comparison with normal renal tissue (p=0.012) and was associated with high OPN mRNA level, and with NF-κB activation (p<0.001 and p=0.045, respectively). Immunohistochemical staining showed the inverse association; high EGFR protein expression was related with low OPN and NF-κB protein expression (p<0.001 and p=0.047, respectively). Conclusion: Epidermal growth factor receptor gene is upregulated in CRCC and associated with OPN gene expression and NF-kB signaling. The inverse relation between OPN and EGFR at the protein level could probably reflect dynamic changes that EGFR undergoes following activation. Federación de Sociedades Españolas de Oncología (FESEO), 2012 Renal cell carcinoma nationallicence EGFR nationallicence Nuclear factor kappa B nationallicence Osteopontin nationallicence Tissue array analysis nationallicence Matušan-Ilijaš K. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut Damante G. Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy aut Fabbro D. University Hospital Santa Maria della Misericordia, Udine, Italy aut Đorđević G. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut Hadžisejdić I. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut Grahovac M. Department of Dermatology, Ludwig-Maximilians-University Munich, Muenchen, Germany aut Avirović M. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut Grahovac B. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut Jonjić N. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut Lučin K. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut Clinical and Translational Oncology Springer Milan 15/1(2013-01-01), 65-71 1699-048X 15:1<65 2013 15 12094 https://doi.org/10.1007/s12094-012-0889-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-012-0889-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matušan-Ilijaš K. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut NATIONALLICENCE 700 1- Damante G. Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy aut NATIONALLICENCE 700 1- Fabbro D. University Hospital Santa Maria della Misericordia, Udine, Italy aut NATIONALLICENCE 700 1- Đorđević G. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut NATIONALLICENCE 700 1- Hadžisejdić I. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut NATIONALLICENCE 700 1- Grahovac M. Department of Dermatology, Ludwig-Maximilians-University Munich, Muenchen, Germany aut NATIONALLICENCE 700 1- Avirović M. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut NATIONALLICENCE 700 1- Grahovac B. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut NATIONALLICENCE 700 1- Jonjić N. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut NATIONALLICENCE 700 1- Lučin K. Department of Pathology, Rijeka University School of Medicine, Braće Branchetta 20, 51 000, Rijeka, Croatia aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/1(2013-01-01), 65-71 1699-048X 15:1<65 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer