naa a22 4500 510763294 CHVBK 20180411083147.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s12094-012-0959-z doi (NATIONALLICENCE)springer-10.1007/s12094-012-0959-z A novel peptide (Thx) homing to non-small cell lung cancer identified by ex vivo phage display [Elektronische Daten] [A. Koivistoinen, I. Ilonen, K. Punakivi, J. Räsänen, H. Helin, E. Sihvo, M. Bergman, J. Salo] Aim: To identify linear peptide homing to non-small cell lung cancer (NSCLC) tumor cells using ex vivo phage display method. Materials and methods: Twenty-six clinical patient samples were used to identify linear homing peptide, which was exposed to NSCLC cell cultures and control cell lines to determine cell binding affinity and cell localization. Also, ex vivo biodistribution was analyzed using tumor-bearing mice. Results: The panning yielded peptide enrichment with a core motif A/SRXPXXX. Based on this, an amino acid sequence, ARRPKLD, was selected for characterization and named Thx-peptide. The in vitro binding properties of Thx-peptide demonstrated selectivity toward NSCLC. Internalization assays showed that Thx-Alexa and fluorescein conjugates were located in a subset of perinuclearly located lysosomes of tumor cells. Thx-peptide appeared with fluorescein-labeled peptide and peptide-DTPA-chelator complex in adenocarcinoma xenografts in mice. Conclusion: Thx shows promise for targeted imaging and drug delivery. Federación de Sociedades Españolas de Oncología (FESEO), 2012 Lung neoplasms nationallicence Phage display nationallicence Peptides nationallicence Tumor targeting nationallicence Biodistribution nationallicence Koivistoinen A. Karyon-CTT Ltd., Helsinki, Finland aut Ilonen I. Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Haartmaninkatu 4, PO Box 340, 00290, Helsinki, Finland aut Punakivi K. Karyon-CTT Ltd., Helsinki, Finland aut Räsänen J. Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Haartmaninkatu 4, PO Box 340, 00290, Helsinki, Finland aut Helin H. Department of Pathology, Helsinki University Central Hospital, Helsinki, Finland aut Sihvo E. Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Haartmaninkatu 4, PO Box 340, 00290, Helsinki, Finland aut Bergman M. Karyon-CTT Ltd., Helsinki, Finland aut Salo J. Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Haartmaninkatu 4, PO Box 340, 00290, Helsinki, Finland aut Clinical and Translational Oncology Springer Milan 15/6(2013-06-01), 492-498 1699-048X 15:6<492 2013 15 12094 https://doi.org/10.1007/s12094-012-0959-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-012-0959-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koivistoinen A. Karyon-CTT Ltd., Helsinki, Finland aut NATIONALLICENCE 700 1- Ilonen I. Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Haartmaninkatu 4, PO Box 340, 00290, Helsinki, Finland aut NATIONALLICENCE 700 1- Punakivi K. Karyon-CTT Ltd., Helsinki, Finland aut NATIONALLICENCE 700 1- Räsänen J. Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Haartmaninkatu 4, PO Box 340, 00290, Helsinki, Finland aut NATIONALLICENCE 700 1- Helin H. Department of Pathology, Helsinki University Central Hospital, Helsinki, Finland aut NATIONALLICENCE 700 1- Sihvo E. Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Haartmaninkatu 4, PO Box 340, 00290, Helsinki, Finland aut NATIONALLICENCE 700 1- Bergman M. Karyon-CTT Ltd., Helsinki, Finland aut NATIONALLICENCE 700 1- Salo J. Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Haartmaninkatu 4, PO Box 340, 00290, Helsinki, Finland aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/6(2013-06-01), 492-498 1699-048X 15:6<492 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer