naa a22 4500 510763383 CHVBK 20180411083147.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12094-012-0999-4 doi (NATIONALLICENCE)springer-10.1007/s12094-012-0999-4 Resveratrol induces apoptosis of pancreatic cancers cells by inhibiting miR-21 regulation of BCL-2 expression [Elektronische Daten] [P. Liu, H. Liang, Q. Xia, P. Li, H. Kong, P. Lei, S. Wang, Z. Tu] Objective: Resveratrol is an edible polyphenolic phytoalexin present in different plant species and plays important role in inhibiting proliferation and inducing apoptosis of pancreatic cancer cells. In this paper, the mechanism of resveratrol on PANC-1, CFPAC-1 and MIA Paca-2 cells apoptosis was examined. Methods: We first evaluated the effect of resveratrol on viability of PANC-1, CFPAC-1 and MIA Paca-2 cells using MTT assay. Next, we performed real-time PCR to assess the effect of resveratrol on miR-21 expression. We also used Western blot to measure BCL-2 protein levels after down-regulation of miR-21 expression. Finally, we evaluated the effect of miR-21 on resveratrol-induced anti-tumor activity using miR-21 mimic. Results: Resveratrol induced a significant inhibition of PANC-1, CFPAC-1 and MIA Paca-2 cells viability in a dose-dependent manner. Resveratrol also decreased the expression of miR-21. Besides, down-regulation of miR-21 expression can inhibit BCL-2 expression in PANC-1, CFPAC-1 and MIA Paca-2 cells. Over-expression of miR-21 expression can reverse down-regulation of BCL-2 expression and apoptosis induced by resveratrol. Conclusions: In this study, we demonstrated that the effect of resveratrol on apoptosis is due to inhibiting miR-21 regulation of BCL-2 expression. Federación de Sociedades Españolas de Oncología (FESEO), 2013 Pancreatic cancer nationallicence Resveratrol nationallicence miR-21 nationallicence BCL-2 nationallicence Apoptosis nationallicence Liu P. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut Liang H. Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China aut Xia Q. The Faculty of Laboratory Medicine and Tropical Medicine, Hainan Medical College, Haikou, China aut Li P. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut Kong H. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut Lei P. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut Wang S. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut Tu Z. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut Clinical and Translational Oncology Springer Milan 15/9(2013-09-01), 741-746 1699-048X 15:9<741 2013 15 12094 https://doi.org/10.1007/s12094-012-0999-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-012-0999-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu P. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut NATIONALLICENCE 700 1- Liang H. Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China aut NATIONALLICENCE 700 1- Xia Q. The Faculty of Laboratory Medicine and Tropical Medicine, Hainan Medical College, Haikou, China aut NATIONALLICENCE 700 1- Li P. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut NATIONALLICENCE 700 1- Kong H. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut NATIONALLICENCE 700 1- Lei P. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut NATIONALLICENCE 700 1- Wang S. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut NATIONALLICENCE 700 1- Tu Z. Department of Medical Laboratory, Chongqing University of Medical Science, Chongqing, China aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/9(2013-09-01), 741-746 1699-048X 15:9<741 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer