naa a22 4500 510763421 CHVBK 20180411083147.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12094-012-0994-9 doi (NATIONALLICENCE)springer-10.1007/s12094-012-0994-9 Carnosol, radiation and melanoma: a translational possibility [Elektronische Daten] [M. Alcaraz, D. Achel, A. Olivares, E. Olmos, M. Alcaraz-Saura, J. Castillo] Purpose: To compare the genoprotective and radioprotective effect of carnosol (COL) against damage induced by ionizing radiation with similar effects produced by different antioxidant compounds. Methods: The genoprotective effect was studied by means of the micronucleus test for antimutagenic activity in which the reduction in the frequency of micronuclei was evaluated in cytokinesis-blocked cells of human lymphocytes. The radioprotective effects were studied by cell viability test (MTT) in PNT2 (normal prostate) and B16F10 (melanoma) cell lines when they were administered before exposure to different X-ray doses (4, 6, 8, 10 and 0Gy). Results: Carnosol shows a significant genoprotective capacity (p<0.001) against radiation with a protection factor of 50%, and a dose-reduction factor of 4.3. Cell survival obtained with COL administered before exposure to 10Gy of X-rays showed a protection factor of 55.1%, eliminating 39% of radiation-induced cell death in normal epithelial cells of prostate (PNT2) (p<0.001). However, in the melanoma cell lines (B16F10) assayed, COL acted not as a radioprotector, but as a sensitizing agent increasing the cellular death by 34% (p<0.01) and producing an enhancement ratio of 2.12. Conclusions: Carnosol may be developed as a radioprotective agent in the non-tumoral cells. However, in the B10F16 melanoma cells, melanogenesis is activated by COL leading to redistribution of the enzymatic balances of glutathione and cysteine-lyase production, which could compromise the intracellular redox defence system. This effect appears as an increase in the capacity of ionizing radiation-induced damage, and thus exhibits a paradoxical protective effect of COL on melanoma cells. Federación de Sociedades Españolas de Oncología (FESEO), 2013 Radiation effects nationallicence Carnosol nationallicence Micronuclei nationallicence Radioprotectors nationallicence Radiosensitizers nationallicence Melanoma nationallicence Alcaraz M. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut Achel D. Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon-Accra, Ghana aut Olivares A. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut Olmos E. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut Alcaraz-Saura M. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut Castillo J. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut Clinical and Translational Oncology Springer Milan 15/9(2013-09-01), 712-719 1699-048X 15:9<712 2013 15 12094 https://doi.org/10.1007/s12094-012-0994-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-012-0994-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alcaraz M. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut NATIONALLICENCE 700 1- Achel D. Applied Radiation Biology Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, Legon-Accra, Ghana aut NATIONALLICENCE 700 1- Olivares A. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut NATIONALLICENCE 700 1- Olmos E. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut NATIONALLICENCE 700 1- Alcaraz-Saura M. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut NATIONALLICENCE 700 1- Castillo J. Radiology and Physical Medicine Department, Faculty of Medicine/Dentistry, University of Murcia, 30100 Espinardo, Murcia, Spain aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/9(2013-09-01), 712-719 1699-048X 15:9<712 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer