naa a22 4500 510763472 CHVBK 20180411083148.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12094-012-0961-5 doi (NATIONALLICENCE)springer-10.1007/s12094-012-0961-5 Increased expression of CD24 is associated with tumor progression and prognosis in patients suffering osteosarcoma [Elektronische Daten] [J. Tang, H. Cai, L. Lin, P. Xie, W. Zhong, M. Tang] Objective: As a small heavily glycosylated mucin-like glycosyl-phosphatidylinositol-anchored cell surface protein, CD24 plays an important role in carcinogenesis of various human malignancies. However, its involvement in osteosarcoma is still unclear. The aim of this study was to investigate the expression pattern and the clinical significance of CD24 in human osteosarcoma. Methods: CD24 mRNA and protein expression levels were, respectively, detected by RT-PCR and Western blot assays using 30 pairs of osteosarcoma and noncancerous bone tissues. Then, immunohistochemistry was performed to analyze the association of CD24 expression in 166 osteosarcoma tissues with clinicopathological factors or survival of patients. Results: CD24 expression at mRNA and protein levels were both significantly higher in osteosarcoma tissues than those in corresponding noncancerous bone tissues (both P<0.001). In addition, CD24 protein was positively expressed in 129 of 166 (77.7%) osteosarcoma specimens with a cytoplasmic and membraneous staining, and also increased in the osteosarcoma specimens with advanced clinical stage (P=0.01) and positive distant metastasis (P=0.005). The univariate and multivariate analyses showed that osteosarcoma patients with high CD24 expression had poorer overall and disease-free survival, and high CD24 expression was an independent prognostic factor for both overall and disease-free survival. Conclusion: The aforementioned findings offer convincing evidence for the first time that the increased expression of CD24 is correlated with tumor aggressiveness and tumor metastasis of osteosarcoma, and this molecule is an independent prognostic marker for osteosarcoma patients. Federación de Sociedades Españolas de Oncología (FESEO), 2012 Osteosarcoma nationallicence CD24 nationallicence Expression nationallicence Prognosis nationallicence Overall survival nationallicence Disease-free survival nationallicence Tang J. Orthopedics Department, Xuhui Central Hospital, No. 966, Middle Huaihai Road, 200031, Shanghai, China aut Cai H. Orthopedics Department, Xuhui Central Hospital, No. 966, Middle Huaihai Road, 200031, Shanghai, China aut Lin L. Orthopedics Department, Zhujiang Hospital, The Southern Medical University, No. 253, Gongye Road, Haizhu District, 510282, Guangzhou, China aut Xie P. Orthopaedics Department, Shanghai East Hospital Tongji University, No. 150, Jimo Road, 200120, Shanghai, China aut Zhong W. Shanghai Changhai Hospital Second Military Medical University, No. 168, Changhai Road, 200433, Shanghai, China aut Tang M. Orthopaedics Department, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, No. 600, Yishan Road, 200233, Shanghai, China aut Clinical and Translational Oncology Springer Milan 15/7(2013-07-01), 541-547 1699-048X 15:7<541 2013 15 12094 https://doi.org/10.1007/s12094-012-0961-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-012-0961-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tang J. Orthopedics Department, Xuhui Central Hospital, No. 966, Middle Huaihai Road, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Cai H. Orthopedics Department, Xuhui Central Hospital, No. 966, Middle Huaihai Road, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Lin L. Orthopedics Department, Zhujiang Hospital, The Southern Medical University, No. 253, Gongye Road, Haizhu District, 510282, Guangzhou, China aut NATIONALLICENCE 700 1- Xie P. Orthopaedics Department, Shanghai East Hospital Tongji University, No. 150, Jimo Road, 200120, Shanghai, China aut NATIONALLICENCE 700 1- Zhong W. Shanghai Changhai Hospital Second Military Medical University, No. 168, Changhai Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Tang M. Orthopaedics Department, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, No. 600, Yishan Road, 200233, Shanghai, China aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/7(2013-07-01), 541-547 1699-048X 15:7<541 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer