naa a22 4500 510763480 CHVBK 20180411083148.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12094-012-0966-0 doi (NATIONALLICENCE)springer-10.1007/s12094-012-0966-0 Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer [Elektronische Daten] [G. Huang, H. Hu, X. Xue, S. Shen, E. Gao, G. Guo, X. Shen, X. Zhang] Introduction: PIWI interacting RNAs (piRNAs) are endogenous regulatory non-coding RNAs which are generally expressed in the germline across animal species. The expressions of piRNAs in tumor tissue were rarely reported until now. The aim of this study was to investigate the expression of piRNAs in breast cancer. Materials and methods: Deep sequencing was carried out in four tumor and four matched non-tumor tissues from resected breast cancer cases to screen out differentially expressed piRNAs. Furthermore, the result was evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR) technology in 50 patients with breast cancer and the piRNA expression levels were correlated with clinicopathologic features. Results: Through deep sequencing, six differentially expressed piRNAs were screened out (5 up- and 1 down-regulated) in the four tumor tissues compared with matched non-tumor tissues. Among them, 4 piRNAs (piR-4987, piR-20365, piR-20485 and piR-20582) were confirmed to be up-regulated by realtime RT-PCR in 50 breast cancer (P<0.001). Moreover, up-regulated piR-4987 expression was associated with lymph node positivity (P=0.001). Conclusions: PiRNAs might play a role in breast cancer and act as tumor markers. Federación de Sociedades Españolas de Oncología (FESEO), 2012 PiRNA nationallicence Deep sequencing nationallicence Biomarker nationallicence Breast cancer nationallicence Non-coding RNA nationallicence Carcinogenesis nationallicence Huang G. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut Hu H. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut Xue X. Department of Microbiology and Immunology, Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut Shen S. Department of General Surgery, The Integration of Traditional Chinese and Western Medicine of Wenzhou, 325000, Wenzhou, Zhejiang, China aut Gao E. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut Guo G. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut Shen X. Department of General Surgery, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut Zhang X. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut Clinical and Translational Oncology Springer Milan 15/7(2013-07-01), 563-568 1699-048X 15:7<563 2013 15 12094 https://doi.org/10.1007/s12094-012-0966-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-012-0966-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang G. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Hu H. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Xue X. Department of Microbiology and Immunology, Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Shen S. Department of General Surgery, The Integration of Traditional Chinese and Western Medicine of Wenzhou, 325000, Wenzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Gao E. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Guo G. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Shen X. Department of General Surgery, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Zhang X. Department of Oncology, The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, Zhejiang, China aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/7(2013-07-01), 563-568 1699-048X 15:7<563 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer