naa a22 4500 510763820 CHVBK 20180411083149.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12094-013-1010-8 doi (NATIONALLICENCE)springer-10.1007/s12094-013-1010-8 Expression of p53 upregulated modulator of apoptosis (PUMA) and C-myb in gallbladder adenocarcinoma and their pathological significance [Elektronische Daten] [W. Cai, Q. Li, Z. Yang, X. Miao, Y. Wen, S. Huang, J. Ouyang] Purpose: An increasing number of studies have shown that PUMA and C-myb signaling pathways are involved in various human cancers including colon carcinomas. However, few studies have examined gallbladder cancer specimens, and little is known about the clinical and pathological significance signaling changes may have in gallbladder adenocarcinoma. This study has investigated the expression of PUMA and C-myb in benign and malignant lesions of gallbladder and its pathological significance. Methods: Tissue specimens from 108 gallbladder adenocarcinoma patients, 46 adjacent tissues, 15 cases of adenomatous polyps, and 35 surgical specimens from chronic cholecystitis patients were routinely paraffin embedded and sectioned. PUMA and C-myb expressions were detected with EnVision immunohistochemistry. Results: Positive rates of PUMA and C-myb are significantly higher in gallbladder adenocarcinoma tissues than that in the other three (P<0.01). Gallbladder epithelial cells in PUMA and/or C-myb positive benign cases manifest moderate to severe atypical dysplasia. Positive rates of PUMA and C-myb in well-differentiated tumors with maximum diameterof <2cm and with no lymph node metastasis and invasion of the surrounding tissues are significantly lower than that in those poorly differentiated cases with maximum diameterof ≥2cm, lymph node metastasis, and invasion of the surrounding tissues (P<0.05 or P<0.01). The postoperative survival of patients whose tumor specimens are positive for PUMA and C-myb is significantly shorter than that of those who are negative for both markers (P<0.05 or P<0.01). Conclusions: Our results have demonstrated that PUMA and C-myb positive gallbladder tumors progress rapidly, are prone to metastasis, possess strong invasive ability, and have poor prognosis. Federación de Sociedades Españolas de Oncología (FESEO), 2013 Gallbladder neoplasms nationallicence Gallbladder polyp nationallicence Chronic cholecystitis nationallicence p53 up-regulated modulator of apoptosis nationallicence C-myb nationallicence Immunohistochemsitry nationallicence Cai W. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Li Q. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Yang Z. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Miao X. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Wen Y. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Huang S. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Ouyang J. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut Clinical and Translational Oncology Springer Milan 15/10(2013-10-01), 818-824 1699-048X 15:10<818 2013 15 12094 https://doi.org/10.1007/s12094-013-1010-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-013-1010-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cai W. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Li Q. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Yang Z. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Miao X. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Wen Y. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Huang S. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Ouyang J. Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Number 139, Renmin Road, 410011, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/10(2013-10-01), 818-824 1699-048X 15:10<818 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer