naa a22 4500 510764029 CHVBK 20180411083150.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12094-012-0923-y doi (NATIONALLICENCE)springer-10.1007/s12094-012-0923-y VEGF-A and VEGF-B mRNA expression in gastro-oesophageal cancers [Elektronische Daten] [Cristina Angelescu, Florin Burada, Mihai Ioana, Radu Angelescu, Emil Moraru, Anca Riza, Sanda Marchian, Francisc Mixich, Mihai Cruce, Adrian Săftoiu] Introduction: Angiogenesis is essential for the local growth, invasion and metastasis of the tumours. Vascular endothelial growth factors (VEGFs) play a crucial role in tumour angiogenesis. The aim of our study was to quantify the expression of several VEGF family molecules in human gastro-oesophageal cancers and to analyse possible correlations between genes expression and clinico-pathological features. Materials and methods: Gene expression was quantified in 43 gastro-oesophageal paired samples using qRT-PCR with TaqMan probes specific to VEGF-A, including soluble transcript variants and VEGF-B genes. Results: VEGF-A, including the studied splice variants and VEGF-B mRNAs were expressed in both tumour and peritumour mucosa. The expression of VEGF-A and its isoforms was higher in tumour compared with paired peritumour mucosa, while no significant difference was observed in VEGF-B expression. VEGF-A expression tended to correlate with tumour invasion. Conclusion: VEGF-A has a tendency to over-express in gastro-oesophageal cancers, while VEGF-B does not seem involved in these tumours. Further studies are required to establish the utility of anti-VEGF-A therapy and to find biomarkers for pathogenesis or response to therapy in gastro-oesophageal tumours. Federación de Sociedades Españolas de Oncología (FESEO), 2012 Gastro-oesophageal cancer nationallicence VEGF-A nationallicence VEGF-B nationallicence Isoform nationallicence qRT-PCR nationallicence Angelescu Cristina Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut Burada Florin Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut Ioana Mihai Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut Angelescu Radu Research Center in Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut Moraru Emil Department of General Surgery, University of Medicine and Pharmacy of Craiova, Craiova, Romania aut Riza Anca Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut Marchian Sanda Faculty of Medicine "Victor Papilian”, "Lucian Blaga” University, Sibiu, Romania aut Mixich Francisc Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut Cruce Mihai Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut Săftoiu Adrian Research Center in Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut Clinical and Translational Oncology Springer Milan 15/4(2013-04-01), 313-320 1699-048X 15:4<313 2013 15 12094 https://doi.org/10.1007/s12094-012-0923-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-012-0923-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Angelescu Cristina Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut NATIONALLICENCE 700 1- Burada Florin Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut NATIONALLICENCE 700 1- Ioana Mihai Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut NATIONALLICENCE 700 1- Angelescu Radu Research Center in Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut NATIONALLICENCE 700 1- Moraru Emil Department of General Surgery, University of Medicine and Pharmacy of Craiova, Craiova, Romania aut NATIONALLICENCE 700 1- Riza Anca Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut NATIONALLICENCE 700 1- Marchian Sanda Faculty of Medicine "Victor Papilian”, "Lucian Blaga” University, Sibiu, Romania aut NATIONALLICENCE 700 1- Mixich Francisc Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut NATIONALLICENCE 700 1- Cruce Mihai Laboratory of Human Genomics, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut NATIONALLICENCE 700 1- Săftoiu Adrian Research Center in Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, Bvd. 1 Mai, No. 66, Craiova, Romania aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/4(2013-04-01), 313-320 1699-048X 15:4<313 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer