naa a22 4500 510764347 CHVBK 20180411083151.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12094-012-0894-z doi (NATIONALLICENCE)springer-10.1007/s12094-012-0894-z Methylation in the p53 promoter in epithelial ovarian cancer [Elektronische Daten] [Marcela Chmelarova, E. Krepinska, J. Spacek, J. Laco, M. Beranek, V. Palicka] Objective: Ovarian cancer is a leading cause of death from gynecologic tumors, however, the molecular and especially epigenetic events underlying this transformation are poorly understood. Promoter methylation status of tumor suppressor genes may be associated with transcriptional silencing and tumor progression. It has been shown that methylation of CpG dinucleotides located in the promoter region of p53 is associated with low expression levels of this gene. The aim of this study was to investigate promoter methylation of p53 gene in ovarian cancer by comparison with normal ovarian tissue. Methods: To search for promoter methylation of p53 gene we used methylation-specific PCR (MSP) to compare the methylation status of 66 tissue samples of ovarian cancer with 37 control samples. Results: In our study methylation specific PCR revealed p53 promoter methylation in 34 of 66 (51.5%) of specimens with ovarian cancer. Conclusion: These results indicate that methylation in p53 promoter region may play an important role in carcinogenesis of ovarian cancer and could potentially be used in screening of ovarian cancer, and may have implications for future chemotherapy based on epigenetic changes. Federación de Sociedades Españolas de Oncología (FESEO), 2012 p53 nationallicence Promoter methylation nationallicence Ovarian cancer nationallicence Chmelarova Marcela Institute of Clinical Biochemistry and Diagnostics, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Sokolska 581, 500 05, Hradec Kralove, Czech Republic aut Krepinska E. Department of Obstetrics and Gynecology, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Hradec Kralove, Czech Republic aut Spacek J. Department of Obstetrics and Gynecology, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Hradec Kralove, Czech Republic aut Laco J. The Fingerland Department of Pathology, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Hradec Kralove, Czech Republic aut Beranek M. Institute of Clinical Biochemistry and Diagnostics, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Sokolska 581, 500 05, Hradec Kralove, Czech Republic aut Palicka V. Institute of Clinical Biochemistry and Diagnostics, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Sokolska 581, 500 05, Hradec Kralove, Czech Republic aut Clinical and Translational Oncology Springer Milan 15/2(2013-02-01), 160-163 1699-048X 15:2<160 2013 15 12094 https://doi.org/10.1007/s12094-012-0894-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-012-0894-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chmelarova Marcela Institute of Clinical Biochemistry and Diagnostics, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Sokolska 581, 500 05, Hradec Kralove, Czech Republic aut NATIONALLICENCE 700 1- Krepinska E. Department of Obstetrics and Gynecology, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Hradec Kralove, Czech Republic aut NATIONALLICENCE 700 1- Spacek J. Department of Obstetrics and Gynecology, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Hradec Kralove, Czech Republic aut NATIONALLICENCE 700 1- Laco J. The Fingerland Department of Pathology, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Hradec Kralove, Czech Republic aut NATIONALLICENCE 700 1- Beranek M. Institute of Clinical Biochemistry and Diagnostics, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Sokolska 581, 500 05, Hradec Kralove, Czech Republic aut NATIONALLICENCE 700 1- Palicka V. Institute of Clinical Biochemistry and Diagnostics, Medical Faculty of Charles University and Faculty Hospital Hradec Kralove, Sokolska 581, 500 05, Hradec Kralove, Czech Republic aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/2(2013-02-01), 160-163 1699-048X 15:2<160 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer