naa a22 4500 510764428 CHVBK 20180411083151.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12094-012-0896-x doi (NATIONALLICENCE)springer-10.1007/s12094-012-0896-x Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells [Elektronische Daten] [Virginia Pistone Creydt, Sabrina Fletcher, Jimena Giudice, Ariana Bruzzone, Norma Chasseing, Eduardo Gonzalez, Paula Sacca, Juan Calvo] Introduction: Stromal-epithelial interactions mediate both breast development and breast cancer progression. In the present work, we evaluated the effects of conditioned media (CMs) of human adipose tissue explants from normal (hATN) and tumor (hATT) breast on proliferation, adhesion, migration and metalloproteases activity on tumor (MCF-7 and IBH-7) and non-tumor (MCF-10A) human breast epithelial cell lines. Materials and methods: Human adipose tissues were obtained from patients and the conditioned medium from hATN and hATT collected after 24h of incubation. MCF-10A, MCF-7 and IBH-7 cells were grown and incubated with CMs and proliferation and adhesion, as well as migration ability and metalloprotease activity, of epithelial cells after exposing cell cultures to hATN- or hATT-CMs were quantified. The statistical significance between different experimental conditions was evaluated by one-way ANOVA. Tukey′s post hoc tests were performed. Results: Tumor and non-tumor breast epithelial cells significantly increased their proliferation activity after 24h of treatment with hATT-CMs compared to control-CMs. Furthermore, cellular adhesion of these two tumor cell lines was significantly lower with hATT-CMs than with hATN-CMs. Therefore, hATT-CMs seem to induce significantly lower expression or less activity of the components involved in cellular adhesion than hATN-CMs. In addition, hATT-CMs induced pro-MMP-9 and MMP-9 activity and increased the migration of MCF-7 and IBH-7 cells compared to hATN-CMs. Conclusions: We conclude that the microenvironment of the tumor interacts in a dynamic way with the mutated epithelium. This evidence leads to the possibility to modify the tumor behavior/phenotype through the regulation or modification of its microenvironment. We developed a model in which we obtained CMs from adipose tissue explants completely, either from normal or tumor breast. In this way, we studied the contribution of soluble factors independently of the possible effects of direct cell contact. Federación de Sociedades Españolas de Oncología (FESEO), 2012 Human breast adipose tissue nationallicence Breast epithelial cells nationallicence Cancer nationallicence Epithelial-stromal interactions nationallicence Pistone Creydt Virginia Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut Fletcher Sabrina Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut Giudice Jimena Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut Bruzzone Ariana Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut Chasseing Norma Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut Gonzalez Eduardo Instituto de Oncología "Ángel H. Roffo”, Universidad de Buenos Aires, Buenos Aires, Argentina aut Sacca Paula Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut Calvo Juan Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut Clinical and Translational Oncology Springer Milan 15/2(2013-02-01), 124-131 1699-048X 15:2<124 2013 15 12094 https://doi.org/10.1007/s12094-012-0896-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-012-0896-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pistone Creydt Virginia Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Fletcher Sabrina Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Giudice Jimena Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Bruzzone Ariana Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Chasseing Norma Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Gonzalez Eduardo Instituto de Oncología "Ángel H. Roffo”, Universidad de Buenos Aires, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Sacca Paula Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Calvo Juan Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428DN, Buenos Aires, Argentina aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/2(2013-02-01), 124-131 1699-048X 15:2<124 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer