naa a22 4500 510764541 CHVBK 20180411083152.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12094-013-1017-1 doi (NATIONALLICENCE)springer-10.1007/s12094-013-1017-1 Gene diagnosis of micrometastases in regional lymph nodes of patients with stage I non-small cell lung cancer: impact on staging and prognosis [Elektronische Daten] [J. Li, Z.-N. Li, L.-C. Yu, S.-B. Shi, L.-P. Ge, J.-R. Wu, Y.-M. Hu] Purpose: The long-term survival of patients with completely resected stage I non-small cell lung cancer (NSCLC) is not optimal because of undetected lymph node micrometastasis at the time of surgery. The aim of this study is to evaluate the role of survivin and livin mRNA expression in histopathologically negative lymph nodes of stage I NSCLC patients as markers of micrometastasis. Methods: Clinical data and tissue samples of primary tumor and lymph nodes were collected from 44 patients with stage I NSCLC. Reverse-transcriptase-PCR (RT-PCR) was used to detect survivin and livin mRNA expression in these tumor and lymph node samples. Results: Survivin mRNA was detected in all tumors, and livin mRNA was detectable in 39 of the 44 primary tumors. The cut-off values of survivin and livin mRNA levels for diagnosing micrometastasis in lymph nodes were set up according to the expression of survivin and livin mRNA in control lymph nodes. Fifteen (34.1%) of 44 stage I NSCCL patients had micrometastasis in lymph nodes by survivin and/or livin mRNA positive expression. Survival analysis showed higher rate of cancer recurrences and tumor-related death in patients with lymph node micrometastasis (P<0.001 and P=0.001, respectively). Tumor-free survival and overall survival were significantly worse in patients with lymph node micrometastasis compared with those without such micrometastasis (P=0.007 and P=0.01, respectively). Conclusion: RT-RCR assay for survivin and livin mRNA can be considered as useful diagnostic tool for the detection of lymph node micrometastasis for stage I NSCLC patients. Federación de Sociedades Españolas de Oncología (FESEO), 2013 Non-small cell lung cancer nationallicence Micrometastasis nationallicence Lymph node nationallicence Survivin nationallicence Livin nationallicence Li J. Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, 438 North Jiefang Rood, 212001, Zhenjiang, China aut Li Z.-N Department of Surgery, Affiliated First Hospital of Nanjing Medical University, Nanjing, China aut Yu L.-C Department of Thoracic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, China aut Shi S.-B Department of Thoracic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, China aut Ge L.-P Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, 438 North Jiefang Rood, 212001, Zhenjiang, China aut Wu J.-R Department of Pathology, Affiliated Hospital of Jiangsu University, Zhenjiang, China aut Hu Y.-M Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, 438 North Jiefang Rood, 212001, Zhenjiang, China aut Clinical and Translational Oncology Springer Milan 15/11(2013-11-01), 882-888 1699-048X 15:11<882 2013 15 12094 https://doi.org/10.1007/s12094-013-1017-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-013-1017-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li J. Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, 438 North Jiefang Rood, 212001, Zhenjiang, China aut NATIONALLICENCE 700 1- Li Z.-N Department of Surgery, Affiliated First Hospital of Nanjing Medical University, Nanjing, China aut NATIONALLICENCE 700 1- Yu L.-C Department of Thoracic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, China aut NATIONALLICENCE 700 1- Shi S.-B Department of Thoracic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, China aut NATIONALLICENCE 700 1- Ge L.-P Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, 438 North Jiefang Rood, 212001, Zhenjiang, China aut NATIONALLICENCE 700 1- Wu J.-R Department of Pathology, Affiliated Hospital of Jiangsu University, Zhenjiang, China aut NATIONALLICENCE 700 1- Hu Y.-M Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, 438 North Jiefang Rood, 212001, Zhenjiang, China aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/11(2013-11-01), 882-888 1699-048X 15:11<882 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer