naa a22 4500 510764592 CHVBK 20180411083152.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12094-013-1073-6 doi (NATIONALLICENCE)springer-10.1007/s12094-013-1073-6 Gut Ivo Centro Nacional de Análisis Genómico, PCB, C/Baldiri Reixac 4, 08028, Barcelona, Spain aut New sequencing technologies [Elektronische Daten] [Ivo Gut] Nucleic acid sequencing is one of the most important tools of biological research with very broad application. Four generations of DNA sequencing technologies can be distinguished by their nature and the kind of output they provide. Sanger sequencing dominated for 30years and was the workhorse of the Human Genome Project. In 2005 the first 2nd generation sequencer was presented with an output orders of magnitude higher than Sanger sequencing and dramatically reduced cost per base. Currently, we are at the dawn of third generation with nanopore systems that are being developed for DNA sequencing. Meanwhile, the field is broadening applications that complement first, second and third generation sequencing systems to get high-resolution genetic information and fourth generation sequencing is on the horizon. Second generation nucleic acid sequencers are systematically applied in many large-scale international projects such as the International Cancer Genome Consortium and the International Human Epigenome Project. Federación de Sociedades Españolas de Oncología (FESEO), 2013 DNA nationallicence Sequencing nationallicence Nucleic acid nationallicence Sequencing technology nationallicence Clinical and Translational Oncology Springer Milan 15/11(2013-11-01), 879-881 1699-048X 15:11<879 2013 15 12094 https://doi.org/10.1007/s12094-013-1073-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-013-1073-6 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Gut Ivo Centro Nacional de Análisis Genómico, PCB, C/Baldiri Reixac 4, 08028, Barcelona, Spain aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/11(2013-11-01), 879-881 1699-048X 15:11<879 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer