naa a22 4500 510764622 CHVBK 20180411083152.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12094-013-1031-3 doi (NATIONALLICENCE)springer-10.1007/s12094-013-1031-3 Assessment of p38α in peripheral blood mononuclear cells (PBMC): a potential blood protein marker of head and neck squamous cell carcinoma [Elektronische Daten] [K. Gill, R. Kumar, B. Mohanti, S. Dey] Purpose: Solid tumors involve an inflammatory microenvironment portrayed by immune cells playing role in cancer progression via inflammatory p38α mitogen-activated protein kinase (MAPK) molecule that produces pro-inflammatory cytokines-TNFα, IL1β and IL6. This study quantified and compared the expression of p38α in peripheral blood mononuclear cells (PBMCs) of HNSCC patients with the healthy subjects. Methods: The PBMC were isolated from the 35 control and 83 HNSCC patients. The expression of p38α in PBMCs was assessed using surface plasmon resonance (SPR), ELISA and western blot analysis. Results: p38α levels were found to be over-expressed in HNSCC patients 0.98ng/μl (95%CI 0.95-1.02) as compared to controls 0.46ng/μl (95%CI 0.42-0.50) (p<0.0001). Conclusion: p38α is over-expressed in PBMCs of HNSCC patients and may play a role in the progression of cancer. This research may translate a protein marker for HNSCC to clinical oncologist for therapeutic intervention and use as a predictive marker. Federación de Sociedades Españolas de Oncología (FESEO), 2013 p38α nationallicence PBMC nationallicence HNSCC nationallicence Translational research nationallicence Gill K. Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India aut Kumar R. Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India aut Mohanti B. Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India aut Dey S. Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India aut Clinical and Translational Oncology Springer Milan 15/11(2013-11-01), 969-973 1699-048X 15:11<969 2013 15 12094 https://doi.org/10.1007/s12094-013-1031-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12094-013-1031-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gill K. Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India aut NATIONALLICENCE 700 1- Kumar R. Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India aut NATIONALLICENCE 700 1- Mohanti B. Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India aut NATIONALLICENCE 700 1- Dey S. Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India aut NATIONALLICENCE 773 0- Clinical and Translational Oncology Springer Milan 15/11(2013-11-01), 969-973 1699-048X 15:11<969 2013 15 12094 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer