naa a22 4500 510769330 CHVBK 20180411083208.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s11818-013-0628-7 doi (NATIONALLICENCE)springer-10.1007/s11818-013-0628-7 Cav2.3E-/R-type voltage-gated calcium channels modulate sleep in mice [Elektronische Daten] [A. Münch, M. Dibué, J. Hescheler, T. Schneider] Modulation des Schlafs durch spannungsgesteuerte Cav2.3-E- und Cav2.3-R-Typ-Kalziumkanäle in Mäusen Mammalian sleep is characterized by cycles of REM and non-REM (NREM), i.e. slow-wave sleep (SWS) phases. The major neuroanatomical basis of SWS is the thalamocortical circuitry, which operates in different functional modes to determine the state of vigilance. At high vigilance, the tonic mode predominates; stages of low vigilance and SWS are characterized by rebound burst firing. Electrophysiologically, rebound bursting depends on low-threshold Ca2 + spikes and T-type Ca2 + channels have been shown to modulate SWS. We recently demonstrated that Cav2.3R-type Ca2 + channels are capable of modulating absence seizures, a pathophysiological aberration of the thalamocortical oscillations related to SWS. We thus analyzed sleep architecture in control and Cav2.3(−|−) mice using implantable electroencephalography (EEG)/electromyography (EMG) radiotelemetry during spontaneous and urethane-induced sleep. The results demonstrate significantly reduced total sleep time and impairment of SWS generation in Cav2.3(−|−) mice, which affects global sleep architecture (i.e. the ratio of REM to NREM). Furthermore, the relative δ power is significantly reduced in Cav2.3(−|−) mice during NREM sleep although these mice display longer prior wakefulness, possibly indicating disturbances in sleep homeostasis. This observation is supported by recordings following urethane administration. This is the first study to shed light on the fundamental role of Cav2.3 channels in rodent sleep physiology. Springer-Verlag Berlin Heidelberg, 2013 Telemetry nationallicence Electroencephalography nationallicence Slow-wave sleep nationallicence REM sleep nationallicence Ion channel nationallicence Telemetrie nationallicence Elektroenzephalographie nationallicence Langsame-Wellen-Schlaf nationallicence REM-Schlaf nationallicence Ionenkanal nationallicence Münch A. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany aut Dibué M. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany aut Hescheler J. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany aut Schneider T. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany aut Somnologie - Schlafforschung und Schlafmedizin Springer Berlin Heidelberg 17/3(2013-09-01), 185-192 1432-9123 17:3<185 2013 17 11818 https://doi.org/10.1007/s11818-013-0628-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11818-013-0628-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Münch A. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany aut NATIONALLICENCE 700 1- Dibué M. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany aut NATIONALLICENCE 700 1- Hescheler J. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany aut NATIONALLICENCE 700 1- Schneider T. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Köln, Germany aut NATIONALLICENCE 773 0- Somnologie - Schlafforschung und Schlafmedizin Springer Berlin Heidelberg 17/3(2013-09-01), 185-192 1432-9123 17:3<185 2013 17 11818 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer