naa a22 4500 510773494 CHVBK 20180411083222.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12022-013-9272-9 doi (NATIONALLICENCE)springer-10.1007/s12022-013-9272-9 The Role of Epithelial Mesenchymal Transition Markers in Thyroid Carcinoma Progression [Elektronische Daten] [Celina Montemayor-Garcia, Heather Hardin, Zhenying Guo, Carolina Larrain, Darya Buehler, Sofia Asioli, Herbert Chen, Ricardo Lloyd] Understanding the molecular mechanisms involved in thyroid cancer progression may provide targets for more effective treatment of aggressive thyroid cancers. Epithelial mesenchymal transition (EMT) is a major pathologic mechanism in tumor progression and is linked to the acquisition of stem-like properties of cancer cells. We examined expression of ZEB1 which activates EMT by binding to the E-box elements in the E-cadherin promoter, and expression of E-cadherin in normal and neoplastic thyroid tissues in a tissue microarray which included 127 neoplasms and 10 normal thyroid specimens. Thyroid follicular adenomas (n = 32), follicular thyroid carcinomas (n = 28), and papillary thyroid carcinomas (n = 57) all expressed E-cadherin and were mostly negative for ZEB1 while most anaplastic thyroid carcinomas (ATC, n = 10) were negative for E-cadherin, but positive for ZEB1. A validation set of 10 whole sections of ATCs showed 90% of cases positive for ZEB1 and all cases were negative for E-cadherin. Analysis of three cell lines (normal thyroid, NTHY-OR13-1; PTC, TPC-1, and ATC, THJ-21T) showed that the ATC cell line expressed the highest levels of ZEB1 while the normal thyroid cell line expressed the highest levels of E-Cadherin. Quantitative RT-PCR analyses showed that Smad7 mRNA was significantly higher in ATC than in any other group (p < 0.05). These results indicate that ATCs show evidence of EMT including decreased expression of E-cadherin and increased expression of ZEB1 compared to well-differentiated thyroid carcinomas and that increased expression of Smad7 may be associated with thyroid tumor progression. Springer Science+Business Media New York, 2013 Thyroid carcinoma nationallicence Anaplastic thyroid carcinoma nationallicence ZEB1 nationallicence Smad7 nationallicence E-Cadherin nationallicence Montemayor-Garcia Celina Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut Hardin Heather Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut Guo Zhenying Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut Larrain Carolina Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut Buehler Darya Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut Asioli Sofia Department of Biomedical Sciences and Human Oncology, University of Turin, Turin, Italy aut Chen Herbert Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA aut Lloyd Ricardo Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut Endocrine Pathology Springer US; http://www.springer-ny.com 24/4(2013-12-01), 206-212 1046-3976 24:4<206 2013 24 12022 https://doi.org/10.1007/s12022-013-9272-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12022-013-9272-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Montemayor-Garcia Celina Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut NATIONALLICENCE 700 1- Hardin Heather Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut NATIONALLICENCE 700 1- Guo Zhenying Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut NATIONALLICENCE 700 1- Larrain Carolina Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut NATIONALLICENCE 700 1- Buehler Darya Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut NATIONALLICENCE 700 1- Asioli Sofia Department of Biomedical Sciences and Human Oncology, University of Turin, Turin, Italy aut NATIONALLICENCE 700 1- Chen Herbert Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA aut NATIONALLICENCE 700 1- Lloyd Ricardo Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, K4/436 Clinical Science Center, 600 Highland Avenue, Box 8550, 53792, Madison, WI, USA aut NATIONALLICENCE 773 0- Endocrine Pathology Springer US; http://www.springer-ny.com 24/4(2013-12-01), 206-212 1046-3976 24:4<206 2013 24 12022 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer