naa a22 4500 510773672 CHVBK 20180411083223.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s12022-013-9244-0 doi (NATIONALLICENCE)springer-10.1007/s12022-013-9244-0 BRAF and RAS Mutations in Follicular Variants of Papillary Thyroid Carcinoma [Elektronische Daten] [Ji Park, Wook Kim, Tae Hwang, Sang Lee, Hyunkyung Kim, Hye Han, So Lim, Wan Kim, Young Yoo, Kyoung Park] Follicular variants of papillary thyroid carcinoma (FVPTC), particularly the encapsulated subtype, often cause a diagnostic dilemma. Therefore, many FVPTCs are interpreted as "indeterminate” in preoperative fine-needle aspiration (FNA). The aim of this study was to analyze the genotypic changes in BRAF codons 600 and 601, as well as the N, H, and KRAS codons 12, 13, and 61 in FVPTCs and investigate the usefulness of preoperative BRAF and RAS mutation analysis as an adjunct diagnostic tool along with routine FNA. Surgically resected thyroid nodules were reviewed to establish the histological diagnosis of FVPTC. All preoperative FNA diagnoses were categorized according to the Bethesda Reporting System. Mutations in BRAF codons 600 and 601, and N, H, KRAS codons 12, 13, and 61 were analyzed by pyrosequencing. Of 132 cases, 81 (61.4%) had a point mutation in one of the BRAF V600E, BRAF K601E, or RAS oncogenes; BRAF V600E in 43(32.6%), BRAF K601E in three (2.3%), and RAS in 35 (26.5%) cases. All mutations were mutually exclusive. Of 78 cases with an FNA indeterminate category diagnosis, 51 (65.4%) were positive for mutations: 24 for BRAF V600E, 3 for BRAF K601E, and 24 for the RAS gene. The KRAS mutation was more frequently found than the HRAS mutation, comprising 22.9% of the RAS mutations, and all KRAS mutations were located at codon 61. This study demonstrated that either BRAF or RAS mutations were present in two thirds of FVPTCs and these mutations were mutually exclusive. Springer Science+Business Media New York, 2013 BRAF nationallicence RAS nationallicence Follicular variant nationallicence Papillary thyroid carcinoma nationallicence Park Ji Department of Pathology, Dongsan Medical Center, Keimyung University School of Medicine, 56 Dalseong-ro, Jung-gu, 700-712, Daegu, Daegu, Republic of Korea aut Kim Wook Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut Hwang Tae Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut Lee Sang Department of Pathology, Dongsan Medical Center, Keimyung University School of Medicine, 56 Dalseong-ro, Jung-gu, 700-712, Daegu, Daegu, Republic of Korea aut Kim Hyunkyung Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut Han Hye Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut Lim So Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut Kim Wan Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut Yoo Young Department of Surgery, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut Park Kyoung Department of Surgery, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut Endocrine Pathology Springer US; http://www.springer-ny.com 24/2(2013-06-01), 69-76 1046-3976 24:2<69 2013 24 12022 https://doi.org/10.1007/s12022-013-9244-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12022-013-9244-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Ji Department of Pathology, Dongsan Medical Center, Keimyung University School of Medicine, 56 Dalseong-ro, Jung-gu, 700-712, Daegu, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Kim Wook Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Hwang Tae Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Lee Sang Department of Pathology, Dongsan Medical Center, Keimyung University School of Medicine, 56 Dalseong-ro, Jung-gu, 700-712, Daegu, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Kim Hyunkyung Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Han Hye Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Lim So Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Kim Wan Department of Pathology, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Yoo Young Department of Surgery, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Park Kyoung Department of Surgery, Konkuk University School of Medicine, 120 Neungdong-ro (Hwayang-dong), Gwangjin-gu, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 773 0- Endocrine Pathology Springer US; http://www.springer-ny.com 24/2(2013-06-01), 69-76 1046-3976 24:2<69 2013 24 12022 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer