naa a22 4500 51077444X CHVBK 20180411083225.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12210-012-0210-4 doi (NATIONALLICENCE)springer-10.1007/s12210-012-0210-4 Role of PGR on in vitro shoot propagation in Cyamopsis tetragonoloba L. (Taub.): a drought tolerant grain legume [Elektronische Daten] [Naseem Ahmad, Mohammad Faisal, Mohammad Anis] An efficient in vitro mass propagation protocol for Cyamopsis tetragonoloba L. (Taub.), a drought tolerant multipurpose grain legume, has been developed. A comparative range of cytokinins have been investigated for multiple shoot induction and plant regeneration with cotyledonary node, node and shoot tip explants. Four weeks exposure with thidiazuron (TDZ) containing medium, prior to transfer to BA supplemented medium was sufficient to induce maximum number of shoots. In descending order regarding shoot formation, the four different cytokinins at the optimum concentration could be ranked as TDZ, BA, Kn and 2iP. Optimum multiple shoot induction occurred in MS medium containing 5.0μM (TDZ) or 10.0μM (BA) where an average of 14.6±0.67 or 7.8±0.73 shoots, respectively, were produced in cotyledonary node explant after 8weeks of incubation. Elongated shoots were rooted on half strength MS medium containing 5.0μM (IBA) with about 45% efficiency, the plantlets thus obtained were transferred to thermocol cups containing sand:soil (3:1) mixture and placed in a hardening room. Survival rate of plantlets in pots was more than 80% and all the regenerated plants appeared morphologically normal. Accademia Nazionale dei Lincei, 2012 Cyamopsis tetragonoloba nationallicence Micropropagation nationallicence Cytokinins nationallicence Multiple shoots nationallicence BA : 6-Benzyladenine Kn Kinetin nationallicence PGRs : Plant growth regulators nationallicence 2iP : 2-Isopentenyl adenine nationallicence TDZ : Thidiazuron nationallicence IBA : Indole-3-butyric acid nationallicence MS : Murashige and Skoog (1962) medium nationallicence NAA : α-Naphthalene acetic acid nationallicence Ahmad Naseem Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, 202 002, Aligarh, India aut Faisal Mohammad Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia aut Anis Mohammad Department of Plant Production, College of Food and Agriculture Sciences, King Saud University, PO Box 2460, 11451, Riyadh, Saudi Arabia aut Rendiconti Lincei Springer Milan 24/1(2013-03-01), 7-12 2037-4631 24:1<7 2013 24 12210 https://doi.org/10.1007/s12210-012-0210-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12210-012-0210-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahmad Naseem Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, 202 002, Aligarh, India aut NATIONALLICENCE 700 1- Faisal Mohammad Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Anis Mohammad Department of Plant Production, College of Food and Agriculture Sciences, King Saud University, PO Box 2460, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 773 0- Rendiconti Lincei Springer Milan 24/1(2013-03-01), 7-12 2037-4631 24:1<7 2013 24 12210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer