naa a22 4500 510774660 CHVBK 20180411083226.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12210-012-0200-6 doi (NATIONALLICENCE)springer-10.1007/s12210-012-0200-6 Vallone Beatrice Dipartimento di Scienze Biochimiche, Università di Roma "La Sapienza”, P.le Aldo Moro, 5, 00185, Rome, Italy aut Time-resolved crystallography for protein structure: the case of heme proteins [Elektronische Daten] [Beatrice Vallone] Time-resolved Laue diffraction is an elegant approach by which polychromatic X-ray pulses are used in combination with rapid laser triggering of light-sensitive samples so as to make a movie of protein reaction dynamics in real time. The current state-of-the-art with protein Laue diffraction has been time-resolved studies of light-induced conformational changes in myoglobin-CO with 150ps resolution. Myoglobin has traversed the whole history of pump-probe Laue diffraction, yielding a massive amount of data that have provided considerable insight into the understanding of protein dynamics. A picture of factors governing myoglobin dynamics following ligand dissociation has been drawn from data arising from wild-type and mutant proteins. Other heme proteins have been studied using this approach: Scapharca inequivalvis dimeric hemoglobin, the FixL PAS domain and the photosynthetic reaction center complex of Blastochloris viridis. Accademia Nazionale dei Lincei, 2012 Time-resolved crystallography nationallicence Laue diffraction nationallicence Protein structural dynamics nationallicence Heme proteins nationallicence Myoglobin nationallicence Rendiconti Lincei Springer Milan 24(2013-02-01), 101-107 2037-4631 24<101 2013 24 12210 https://doi.org/10.1007/s12210-012-0200-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12210-012-0200-6 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Vallone Beatrice Dipartimento di Scienze Biochimiche, Università di Roma "La Sapienza”, P.le Aldo Moro, 5, 00185, Rome, Italy aut NATIONALLICENCE 773 0- Rendiconti Lincei Springer Milan 24(2013-02-01), 101-107 2037-4631 24<101 2013 24 12210 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer