naa a22 4500 510775632 CHVBK 20180411083229.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12975-012-0232-3 doi (NATIONALLICENCE)springer-10.1007/s12975-012-0232-3 DARPP-32 to Quantify Intracerebral Hemorrhage-Induced Neuronal Death in Basal Ganglia [Elektronische Daten] [Hang Jin, Guohua Xi, Richard Keep, Jiang Wu, Ya Hua] Quantification of acute brain injury in basal ganglia is essential for mechanistic and therapeutic studies in experimental intracerebral hemorrhage (ICH). Using conventional counting of degenerating cells based on morphological or immunohistochemical criteria, it is hard to define the boundary of the whole lesion area. Dopamine- and cAMP-regulated phosphoprotein, Mr 32kDa (DARPP-32) is a cytosolic protein highly enriched in medium-sized spiny neurons of the striatum. We developed new methods for quantifying lesion area by detecting the difference of the DARPP-32 negative area and the hematoma clot and by measuring DARPP-32 protein level for semi-qualification in rat model of ICH. We found that DARPP-32-negative area around the hematoma was present at day 1, peaked at day 3, and decreased at day 14 after ICH, a time course paralleled by DARPP-32 Western blots. The DARPP-32-negative area matched well with the necrotic area determined using propidium iodide. Treatment with an iron chelator, deferoxamine, attenuated the ICH-induced reduction in DARPP-32 protein levels. These results suggest that DARPP-32 is a simple and quantifiable indicator of ICH-induced neuronal death in basal ganglia. Springer Science+Business Media New York, 2012 DARPP-32 nationallicence Intracerebral hemorrhage nationallicence Iron nationallicence Neuronal death nationallicence Jin Hang Department of Neurosurgery, University of Michigan, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Xi Guohua Department of Neurosurgery, University of Michigan, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Keep Richard Department of Neurosurgery, University of Michigan, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Wu Jiang Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin, China aut Hua Ya Department of Neurosurgery, University of Michigan, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Translational Stroke Research Springer US; http://www.springer-ny.com 4/1(2013-02-01), 130-134 1868-4483 4:1<130 2013 4 12975 https://doi.org/10.1007/s12975-012-0232-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-012-0232-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Hang Department of Neurosurgery, University of Michigan, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Xi Guohua Department of Neurosurgery, University of Michigan, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Keep Richard Department of Neurosurgery, University of Michigan, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Wu Jiang Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin, China aut NATIONALLICENCE 700 1- Hua Ya Department of Neurosurgery, University of Michigan, 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- Translational Stroke Research Springer US; http://www.springer-ny.com 4/1(2013-02-01), 130-134 1868-4483 4:1<130 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer