naa a22 4500 510775810 CHVBK 20180411083229.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12975-013-0274-1 doi (NATIONALLICENCE)springer-10.1007/s12975-013-0274-1 mRNA Redistribution during Permanent Focal Cerebral Ischemia [Elektronische Daten] [Monique Lewis, Jill Jamison, Joseph Dunbar, Donald DeGracia] Translation arrest occurs in neurons following focal cerebral ischemia and is irreversible in penumbral neurons destined to die. Following global cerebral ischemia, mRNA is sequestered away from 40S ribosomal subunits as mRNA granules, precluding translation. Here, we investigated mRNA granule formation using fluorescence in situ histochemistry out to 8h permanent focal cerebral ischemia using middle cerebral artery occlusion in Long Evans rats with and without diabetes. Neuronal mRNA granules colocalized with PABP, HuR, and NeuN, but not 40S or 60S ribosomal subunits, or organelle markers. The volume of brain with mRNA granule-containing neurons decreased exponentially with ischemia duration, and was zero after 8h permanent focal cerebral ischemia or any duration of ischemia in diabetic rats. These results show that neuronal mRNA granule response has a limited range of insult intensity over which it is expressed. Identifying the limits of effective neuronal stress response to ischemia will be important for developing effective stroke therapies. Springer Science+Business Media New York, 2013 Focal cerebral ischemia nationallicence HSP70 nationallicence HuR nationallicence Middle cerebral artery occlusion nationallicence mRNA granules nationallicence Protein synthesis inhibition nationallicence Stress granules nationallicence Translation arrest nationallicence CA : Cornu Ammonis nationallicence CCA : Common carotid artery nationallicence COX IV : Cytochrome c oxidase subunit IV nationallicence DND : Delayed neuronal death nationallicence eIF2 : Eukaryotic initiation factor-2 nationallicence ER : Endoplasmic reticulum nationallicence FISH : Fluorescent in situ hybridization nationallicence GM130 : cis-Golgi matrix marker 130kDa nationallicence IF : Immunofluorescence histochemistry nationallicence IP : Intraperitoneal nationallicence I/R : Ischemia and reperfusion nationallicence LONI : Laboratory of Neuroimaging at University of California Los Angeles nationallicence MCA : Middle cerebral artery nationallicence MCAO : Middle cerebral artery occlusion nationallicence NeuN : Neuronal nuclei nationallicence NF H/M : Neurofilament heavy and medium weight chains nationallicence pA : Polyadenylated mRNAs nationallicence PABP : Polyadenylated mRNA-binding protein nationallicence PDI : Protein disulfide isomerase nationallicence pMCAO : Permanent MCAO nationallicence RPA : Ribosomal P antigen nationallicence SG : Stress granules nationallicence S6 : Constituent protein of the 40S small ribosomal subunit nationallicence STZ : Streptozocin nationallicence TA : Translation arrest nationallicence TGN38 : trans-Golgi network 38kDa antigen nationallicence TIA-1 : T-cell internal antigen nationallicence TTC : 2,3,5-Triphenyltetrazolium chloride nationallicence TTP : Tristetraprolin nationallicence Lewis Monique Department of Physiology, Wayne State University School of Medicine, 4116 Scott Hall, 540 East Canfield Ave, 48201, Detroit, MI, USA aut Jamison Jill Department of Physiology, Wayne State University School of Medicine, 4116 Scott Hall, 540 East Canfield Ave, 48201, Detroit, MI, USA aut Dunbar Joseph Department of Physiology, Wayne State University School of Medicine, 4116 Scott Hall, 540 East Canfield Ave, 48201, Detroit, MI, USA aut DeGracia Donald Department of Physiology, Wayne State University School of Medicine, 4116 Scott Hall, 540 East Canfield Ave, 48201, Detroit, MI, USA aut Translational Stroke Research Springer US; http://www.springer-ny.com 4/6(2013-12-01), 604-617 1868-4483 4:6<604 2013 4 12975 https://doi.org/10.1007/s12975-013-0274-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-013-0274-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lewis Monique Department of Physiology, Wayne State University School of Medicine, 4116 Scott Hall, 540 East Canfield Ave, 48201, Detroit, MI, USA aut NATIONALLICENCE 700 1- Jamison Jill Department of Physiology, Wayne State University School of Medicine, 4116 Scott Hall, 540 East Canfield Ave, 48201, Detroit, MI, USA aut NATIONALLICENCE 700 1- Dunbar Joseph Department of Physiology, Wayne State University School of Medicine, 4116 Scott Hall, 540 East Canfield Ave, 48201, Detroit, MI, USA aut NATIONALLICENCE 700 1- DeGracia Donald Department of Physiology, Wayne State University School of Medicine, 4116 Scott Hall, 540 East Canfield Ave, 48201, Detroit, MI, USA aut NATIONALLICENCE 773 0- Translational Stroke Research Springer US; http://www.springer-ny.com 4/6(2013-12-01), 604-617 1868-4483 4:6<604 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer