naa a22 4500 510775845 CHVBK 20180411083230.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12975-013-0272-3 doi (NATIONALLICENCE)springer-10.1007/s12975-013-0272-3 Bipyridine, an Iron Chelator, Does Not Lessen Intracerebral Iron-Induced Damage or Improve Outcome After Intracerebral Hemorrhagic Stroke in Rats [Elektronische Daten] [Jayalakshmi Caliaperumal, Shannon Wowk, Sarah Jones, Yonglie Ma, Frederick Colbourne] Iron chelators, such as the intracellular ferrous chelator 2,2′-bipyridine, are a potential means of ameliorating iron-induced injury after intracerebral hemorrhage (ICH). We evaluated bipyridine against the collagenase and whole-blood ICH models and a simplified model of iron-induced damage involving a striatal injection of FeCl2 in adult rats. First, we assessed whether bipyridine (25mg/kg beginning 12h post-ICH and every 12h for 3days) would attenuate non-heme iron levels in the brain and lessen behavioral impairments (neurological deficit scale, corner turn test, and horizontal ladder) 7days after collagenase-induced ICH. Second, we evaluated bipyridine (20mg/kg beginning 6h post-ICH and then every 24h) on edema 3days after collagenase infusion. Body temperature was continually recorded in a subset of these rats beginning 24h prior to ICH until euthanasia. Third, bipyridine was administered (as per experiment 2) after whole-blood infusion to examine tissue loss, neuronal degeneration, and behavioral impairments at 7days post-stroke, as well as body temperature for 3days post-stroke. Finally, we evaluated whether bipyridine (25mg/kg given 2h prior to surgery and then every 12h for 3days) lessens tissue loss, neuronal death, and behavioral deficits after striatal FeCl2 injection. Bipyridine caused a significant hypothermic effect (maximum drop to 34.6°C for 2-5h after each injection) in both ICH models; however, in all experiments bipyridine-treated rats were indistinguishable from vehicle controls on all other measures (e.g., tissue loss, behavioral impairments, etc.). These results do not support the use of bipyridine against ICH. Springer Science+Business Media New York, 2013 Intracerebral hemorrhage nationallicence Bipyridine nationallicence Iron toxicity nationallicence Iron chelater nationallicence Hypothermia nationallicence Stroke nationallicence ICH : Intracerebral hemorrhage nationallicence Bipyridine : 2,2′-Bipyridine nationallicence BIP : Bipyridine group nationallicence SAL : Saline group nationallicence NDS : Neurological deficit scale nationallicence CTT : Corner turn test nationallicence BWC : Brain water content nationallicence Cb : Cerebellum nationallicence Caliaperumal Jayalakshmi Centre for Neuroscience, University of Alberta, Edmonton, Alberta, Canada aut Wowk Shannon Centre for Neuroscience, University of Alberta, Edmonton, Alberta, Canada aut Jones Sarah Department of Psychology, Biological Sciences, University of Alberta, Room P217 Biological Sciences Building, T6G 2E9, Edmonton, Alberta, Canada aut Ma Yonglie Department of Psychology, Biological Sciences, University of Alberta, Room P217 Biological Sciences Building, T6G 2E9, Edmonton, Alberta, Canada aut Colbourne Frederick Centre for Neuroscience, University of Alberta, Edmonton, Alberta, Canada aut Translational Stroke Research Springer US; http://www.springer-ny.com 4/6(2013-12-01), 719-728 1868-4483 4:6<719 2013 4 12975 https://doi.org/10.1007/s12975-013-0272-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-013-0272-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Caliaperumal Jayalakshmi Centre for Neuroscience, University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Wowk Shannon Centre for Neuroscience, University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Jones Sarah Department of Psychology, Biological Sciences, University of Alberta, Room P217 Biological Sciences Building, T6G 2E9, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Ma Yonglie Department of Psychology, Biological Sciences, University of Alberta, Room P217 Biological Sciences Building, T6G 2E9, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Colbourne Frederick Centre for Neuroscience, University of Alberta, Edmonton, Alberta, Canada aut NATIONALLICENCE 773 0- Translational Stroke Research Springer US; http://www.springer-ny.com 4/6(2013-12-01), 719-728 1868-4483 4:6<719 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer