naa a22 4500 510775853 CHVBK 20180411083230.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12975-013-0279-9 doi (NATIONALLICENCE)springer-10.1007/s12975-013-0279-9 Fission and Fusion of the Neuronal Endoplasmic Reticulum [Elektronische Daten] [K. Kucharz, T. Wieloch, H. Toresson] The endoplasmic reticulum (ER) is central for protein synthesis and is the largest intracellular Ca2+ store in neurons. The neuronal ER is classically described to have a continuous lumen spanning all cellular compartments. This allows neuronal ER to integrate spatially separate events in the cell. Recent in vitro as well as in vivo findings, however, demonstrate that the neuronal ER is a structurally dynamic entity, capable of rapid fragmentation, i.e., ER fission. The ER fragments can fuse back together and reinstate ER continuity. This reversible phenomenon can be induced repeatedly within the same cell, is temperature-dependent, and compatible with cell survival. The key trigger for dendritic ER fission is N-methyl D-aspartate (NMDA) receptor stimulation in the presence of extracellular Ca2+. However, the exact molecular machinery responsible for the fission and fusion of neuronal ER remains unknown. Reversible ER fission represents a new cell biological event downstream of NMDA receptor-gated Ca2+ influx and may thus influence many aspects of neuronal function in physiology and disease. Hence, it constitutes a new field for exploration in neuroscience that will benefit greatly from recent advances in light microscopy imaging techniques allowing dynamic characterization of cellular events in vitro and in vivo. Springer Science+Business Media New York, 2013 Endoplasmic reticulum nationallicence Structure nationallicence Function nationallicence Fragmentation nationallicence Fission nationallicence Continuity nationallicence NMDAR nationallicence Calcium nationallicence Excitotoxicity nationallicence Ischemia nationallicence Kucharz K. Faculty of Health and Medical Sciences, Neuronal Signaling Laboratory, University of Copenhagen, Copenhagen, Denmark aut Wieloch T. Faculty of Medicine, Laboratory for Experimental Brain Research, Lund University, Lund, Sweden aut Toresson H. Skåne University Hospital, Clinical Memory Research Unit, Lund University, Malmö, Sweden aut Translational Stroke Research Springer US; http://www.springer-ny.com 4/6(2013-12-01), 652-662 1868-4483 4:6<652 2013 4 12975 https://doi.org/10.1007/s12975-013-0279-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-013-0279-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kucharz K. Faculty of Health and Medical Sciences, Neuronal Signaling Laboratory, University of Copenhagen, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Wieloch T. Faculty of Medicine, Laboratory for Experimental Brain Research, Lund University, Lund, Sweden aut NATIONALLICENCE 700 1- Toresson H. Skåne University Hospital, Clinical Memory Research Unit, Lund University, Malmö, Sweden aut NATIONALLICENCE 773 0- Translational Stroke Research Springer US; http://www.springer-ny.com 4/6(2013-12-01), 652-662 1868-4483 4:6<652 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer