naa a22 4500 510776140 CHVBK 20180411083230.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12975-013-0268-z doi (NATIONALLICENCE)springer-10.1007/s12975-013-0268-z Phenotypic Changes in Immune Cell Subsets Reflect Increased Infarct Volume in Male vs. Female Mice [Elektronische Daten] [Anirban Banerjee, Jianming Wang, Sheetal Bodhankar, Arthur Vandenbark, Stephanie Murphy, Halina Offner] Inflammatory responses in the brain after cerebral ischemia have been studied extensively in male mice, but not female mice, thus potentially giving a less-than-accurate view of gender associated pathological processes. In humans, cerebral infarcts are typically smaller in premenopausal females than in age-matched males. In the current study, we confirmed smaller infarcts in female vs. male mice after middle cerebral artery occlusion and 96h of reperfusion. Moreover, we explored immunological alterations related to this difference and found that the percentage of CD4+ T lymphocytes was significantly higher in spleens in males than females, with increased expression of the activation markers, CD69 and CD44. In contrast, the percentage of CD8+ T lymphocytes was significantly higher in spleens of females than males, leading to the identification of a small but distinct population of IL-10-secreting CD8+CD122+ suppressor T cells that were also increased in females. Finally, we observed that males have a greater percentage of activated macrophages/microglia in the brain than females, as well as increased expression of the VLA-4 adhesion molecule in both brain and spleen. This new information suggesting gender-dependent immunological mechanisms in stroke implies that effective treatments for human stroke may also be gender specific. Springer Science+Business Media New York, 2013 Experimental stroke nationallicence Gender bias nationallicence Immune markers nationallicence Activated T cells nationallicence Suppressor T cells nationallicence Ischemia nationallicence Banerjee Anirban Neuroimmunology Research, Portland Veterans Affairs Medical Center, R&D-31, 3710 SW US Veterans Hospital Road, Portland, OR, USA aut Wang Jianming Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA aut Bodhankar Sheetal Neuroimmunology Research, Portland Veterans Affairs Medical Center, R&D-31, 3710 SW US Veterans Hospital Road, Portland, OR, USA aut Vandenbark Arthur Neuroimmunology Research, Portland Veterans Affairs Medical Center, R&D-31, 3710 SW US Veterans Hospital Road, Portland, OR, USA aut Murphy Stephanie Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA aut Offner Halina Neuroimmunology Research, Portland Veterans Affairs Medical Center, R&D-31, 3710 SW US Veterans Hospital Road, Portland, OR, USA aut Translational Stroke Research Springer US; http://www.springer-ny.com 4/5(2013-10-01), 554-563 1868-4483 4:5<554 2013 4 12975 https://doi.org/10.1007/s12975-013-0268-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-013-0268-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Banerjee Anirban Neuroimmunology Research, Portland Veterans Affairs Medical Center, R&D-31, 3710 SW US Veterans Hospital Road, Portland, OR, USA aut NATIONALLICENCE 700 1- Wang Jianming Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA aut NATIONALLICENCE 700 1- Bodhankar Sheetal Neuroimmunology Research, Portland Veterans Affairs Medical Center, R&D-31, 3710 SW US Veterans Hospital Road, Portland, OR, USA aut NATIONALLICENCE 700 1- Vandenbark Arthur Neuroimmunology Research, Portland Veterans Affairs Medical Center, R&D-31, 3710 SW US Veterans Hospital Road, Portland, OR, USA aut NATIONALLICENCE 700 1- Murphy Stephanie Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA aut NATIONALLICENCE 700 1- Offner Halina Neuroimmunology Research, Portland Veterans Affairs Medical Center, R&D-31, 3710 SW US Veterans Hospital Road, Portland, OR, USA aut NATIONALLICENCE 773 0- Translational Stroke Research Springer US; http://www.springer-ny.com 4/5(2013-10-01), 554-563 1868-4483 4:5<554 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer