naa a22 4500 510776191 CHVBK 20180411083231.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12975-013-0262-5 doi (NATIONALLICENCE)springer-10.1007/s12975-013-0262-5 A Model of Rat Embolic Cerebral Infarction with a Quantifiable, Autologous Arterial Blood Clot [Elektronische Daten] [Norihito Shimamura, Naoya Matsuda, Kiyohide Kakuta, Akiko Narita, Hiroki Ohkuma] We developed a novel model of a rat embolic cerebral infarction with a quantifiable autologous arterial blood clot. The left femoral artery had 0.15ml of blood withdrawn and mixed with 10units of thrombin in 50μl saline. After 30min, the clot was suctioned into a 4-French polyvinyl chloride tube. A 24-gage catheter was inserted up through the internal carotid artery via the external carotid artery stump. The 1-cm clot, at a volume of 7.2mm3, was pushed and inserted into the internal carotid artery via the catheter. After withdrawing the catheter, the ICA blood flow recovered. We checked neurological status after 24h (neurological free was 15, and worst was 1) and measured the infarction volume by the TTC method. Twelve rats were examined, and five sham-operated rats were included. Two rats were not able to achieve an 80% reduction in CBF. One rat died due to cerebral infarction. The success rate in producing infarction was 83%. The total infarction volume was 368.5mm3 ± 61.2se. Median neurological score was 6. Hemorrhagic transformation was not detected. Sham-operated rats revealed no infarction and no neurological deficit. The volume of infarction correlated significantly with the neurological score. We conclude that this embolic stroke model is useful in producing a human, severe cardioembolic cerebral infarction. Springer Science+Business Media New York, 2013 Autologous arterial clot nationallicence Embolic cerebral ischemia nationallicence Pathophysiology nationallicence Quantifiable clot nationallicence Rat nationallicence Shimamura Norihito Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut Matsuda Naoya Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut Kakuta Kiyohide Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut Narita Akiko Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut Ohkuma Hiroki Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut Translational Stroke Research Springer US; http://www.springer-ny.com 4/5(2013-10-01), 564-570 1868-4483 4:5<564 2013 4 12975 https://doi.org/10.1007/s12975-013-0262-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-013-0262-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shimamura Norihito Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut NATIONALLICENCE 700 1- Matsuda Naoya Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut NATIONALLICENCE 700 1- Kakuta Kiyohide Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut NATIONALLICENCE 700 1- Narita Akiko Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut NATIONALLICENCE 700 1- Ohkuma Hiroki Department of Neurosurgery, Hirosaki University School of Medicine, 5-Zaihuchou, 036-8562, Hirosaki, Aomori Prefecture, Japan aut NATIONALLICENCE 773 0- Translational Stroke Research Springer US; http://www.springer-ny.com 4/5(2013-10-01), 564-570 1868-4483 4:5<564 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer