naa a22 4500 510776213 CHVBK 20180411083231.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12975-013-0270-5 doi (NATIONALLICENCE)springer-10.1007/s12975-013-0270-5 Deferoxamine Reduces Neuronal Death and Hematoma Lysis After Intracerebral Hemorrhage in Aged Rats [Elektronische Daten] [Tetsuhiro Hatakeyama, Masanobu Okauchi, Ya Hua, Richard Keep, Guohua Xi] Intracerebral hemorrhage (ICH) is primarily a disease of the elderly. Deferoxamine (DFX), an iron chelator, reduces long-term neurological deficits and brain atrophy after ICH in aged rats. In the present study, we investigated whether DFX can reduce acute ICH-induced neuronal death and whether it affects the endogenous response to ICH (ferritin upregulation and hematoma resolution) in aged rats. Male Fischer 344 rats (18months old) had an intracaudate injection of 100μL autologous whole blood into the right basal ganglia and were treated with DFX (100mg/kg) or vehicle 2h post-ICH and then every 12h up to 7days. Rats were euthanized 1, 3, or 7days later for neuronal death and ferritin and hematoma size measurements. Plasma ferritin levels and behavioral outcome following ICH were also examined. DFX treatment significantly reduced ICH-induced neuronal death and neurological deficits. DFX also suppressed ferritin upregulation in the ipsilateral basal ganglia after ICH and hematoma lysis (hematoma volume at day7, 13.2 ± 4.9 vs. 3.8 ± 1.2mm3 in vehicle-treated group, p < 0.01). However, effects of DFX on plasma ferritin levels after ICH did not reach significance. In conclusion, DFX reduces neuronal death and neurological deficits after ICH in aged rats. It also affects the endogenous response to ICH. Springer Science+Business Media New York, 2013 Cerebral hemorrhage nationallicence Deferoxamine nationallicence Iron nationallicence Neuronal death nationallicence Hatakeyama Tetsuhiro Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Okauchi Masanobu Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Hua Ya Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Keep Richard Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Xi Guohua Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut Translational Stroke Research Springer US; http://www.springer-ny.com 4/5(2013-10-01), 546-553 1868-4483 4:5<546 2013 4 12975 https://doi.org/10.1007/s12975-013-0270-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-013-0270-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hatakeyama Tetsuhiro Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Okauchi Masanobu Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Hua Ya Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Keep Richard Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Xi Guohua Department of Neurosurgery, University of Michigan, R5018, BSRB 109 Zina Pitcher Place, 48109-2200, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- Translational Stroke Research Springer US; http://www.springer-ny.com 4/5(2013-10-01), 546-553 1868-4483 4:5<546 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer