naa a22 4500 510776302 CHVBK 20180411083231.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12975-012-0250-1 doi (NATIONALLICENCE)springer-10.1007/s12975-012-0250-1 Differentiation of Oligodendrocytes from Mouse Induced Pluripotent Stem Cells Without Serum [Elektronische Daten] [Sachiyo Misumi, Ruriko Nishigaki, Yoshitomo Ueda, Yoko Watanabe, Yuko Shimizu, Akimasa Ishida, ChaGyun Jung, Hideki Hida] Cell therapy using induced pluripotent stem (iPS) cells might become a new approach for treating neonatal hypoxic-ischemic injury such as periventricular leukomalacia. To obtain appropriate donor cells for transplantation, we differentiated oligodendrocyte (OL) lineage cells from mouse iPS cells. Induction of OL lineage cell differentiation from iPS cells was carried out with a seven-step culture method. Mouse iPS cells (stage 1) were induced to form embryoid bodies for 4days under a serum-free condition that was suitable for ectoderm induction (stage 2), following by selection of nestin-positive neural stem cells (NSCs) for 10-12days (stage 3). NSCs were cultured in expansion medium containing fibroblast growth factor (FGF)-2 for 4days (stage 4), induced to differentiate into glial progenitor cells by epidermal growth factor and fibroblast growth factor (FGF-2) treatment for 4-5days (stage 5), and then into OL progenitor cells by culture in neurobasal A medium containing FGF-2 and platelet-derived growth factor for 6-8days (stage 6). Terminal differentiation into O4-positive OLs was carried out by culture in neurobasal A containing T3 and ciliary neurotrophic factor for 7days (stage 7). Inwardly rectifying K+ currents, which are characteristic of OLs, were detected in iPS cell-derived cells at stage 7 in whole cell clamp mode. Our data suggest that OLs can be effectively differentiated from mouse iPS cells without serum in a stepwise manner, which may be appropriate for use as donor cells in transplantation. Springer Science+Business Media New York, 2013 Periventricular leukomalacia nationallicence Donor cells nationallicence Embryoid body nationallicence Inwardly rectifying current nationallicence O4 antigen nationallicence Misumi Sachiyo Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut Nishigaki Ruriko Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut Ueda Yoshitomo Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut Watanabe Yoko Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut Shimizu Yuko Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut Ishida Akimasa Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut Jung ChaGyun Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut Hida Hideki Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut Translational Stroke Research Springer-Verlag 4/2(2013-04-01), 149-157 1868-4483 4:2<149 2013 4 12975 https://doi.org/10.1007/s12975-012-0250-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-012-0250-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Misumi Sachiyo Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Nishigaki Ruriko Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Ueda Yoshitomo Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Watanabe Yoko Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Shimizu Yuko Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Ishida Akimasa Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Jung ChaGyun Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Hida Hideki Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan aut NATIONALLICENCE 773 0- Translational Stroke Research Springer-Verlag 4/2(2013-04-01), 149-157 1868-4483 4:2<149 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer