naa a22 4500 510776337 CHVBK 20180411083231.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12975-012-0217-2 doi (NATIONALLICENCE)springer-10.1007/s12975-012-0217-2 Inhaled Nitric Oxide Protects Males But not Females from Neonatal Mouse Hypoxia-Ischemia Brain Injury [Elektronische Daten] [Changlian Zhu, Yanyan Sun, Jianfeng Gao, Xiaoyang Wang, Nikolaus Plesnila, Klas Blomgren] It was recently discovered that while under normal conditions inhaled nitric oxide (iNO) does not affect cerebral blood flow, it selectively dilates arterioles in the ischemic penumbra during experimental cerebral ischemia, thereby increasing collateral blood flow and reducing ischemic brain damage. The mechanism was verified in multiple models, but only in male animals. Our aim was to evaluate the effects of iNO on brain injury in neonatal males and females. Nine-day-old mice were subjected to unilateral hypoxia-ischemia (HI), using 10% oxygen balanced with nitrogen, with or without 50ppm NO. Brain injury 72h after HI was reduced by iNO as judged by percentage of injury (−21.7%), atrophy (−23.7%), and total pathological score (−29%). The injury was significantly reduced in males (−32.4%, p < 0.05) but not in females (−7.1%, n.s.). Neither the numbers nor the proliferation rates of neural stem cells in the dentate gyrus were affected by iNO. In summary, intraischemic iNO reduced neonatal HI brain injury in a gender-related manner. Springer Science+Business Media New York, 2012 Gender difference nationallicence Asphyxia nationallicence Neonate nationallicence Neuroprotection nationallicence Nitric oxide nationallicence Microglia nationallicence Zhu Changlian Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Box 432, SE 405 30, Göteborg, Sweden aut Sun Yanyan Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Box 432, SE 405 30, Göteborg, Sweden aut Gao Jianfeng Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Box 432, SE 405 30, Göteborg, Sweden aut Wang Xiaoyang Department of Pediatrics, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China aut Plesnila Nikolaus Institute for Stroke and Dementia Research, University of Munich Medical School, Munich, Germany aut Blomgren Klas Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Box 432, SE 405 30, Göteborg, Sweden aut Translational Stroke Research Springer-Verlag 4/2(2013-04-01), 201-207 1868-4483 4:2<201 2013 4 12975 https://doi.org/10.1007/s12975-012-0217-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-012-0217-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Changlian Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Box 432, SE 405 30, Göteborg, Sweden aut NATIONALLICENCE 700 1- Sun Yanyan Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Box 432, SE 405 30, Göteborg, Sweden aut NATIONALLICENCE 700 1- Gao Jianfeng Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Box 432, SE 405 30, Göteborg, Sweden aut NATIONALLICENCE 700 1- Wang Xiaoyang Department of Pediatrics, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China aut NATIONALLICENCE 700 1- Plesnila Nikolaus Institute for Stroke and Dementia Research, University of Munich Medical School, Munich, Germany aut NATIONALLICENCE 700 1- Blomgren Klas Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Box 432, SE 405 30, Göteborg, Sweden aut NATIONALLICENCE 773 0- Translational Stroke Research Springer-Verlag 4/2(2013-04-01), 201-207 1868-4483 4:2<201 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer