naa a22 4500 510776361 CHVBK 20180411083231.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12975-012-0225-2 doi (NATIONALLICENCE)springer-10.1007/s12975-012-0225-2 Granulocyte-colony Stimulating Factor in Combination with Stem Cell Factor Confers Greater Neuroprotection after Hypoxic-Ischemic Brain Damage in the Neonatal Rats than a Solitary Treatment [Elektronische Daten] [Desislava Doycheva, Gary Shih, Hank Chen, Richard Applegate, John Zhang, Jiping Tang] Neonatal hypoxia-ischemia (HI) is a devastating condition resulting in neuronal cell death and often culminates in neurological deficits. Granulocyte-colony stimulating factor (G-CSF) has been shown to have neuroprotective activity via inhibition of apoptosis and inflammation in various stroke models. Stem cell factor (SCF) regulates hematopoietic stem cells in the bone marrow and has been reported to have neuroprotective properties in an experimental ischemic stroke model. In this study, we aim to determine the protective effects of G-CSF in combination with SCF treatment after experimental HI. Seven-day-old Sprague-Dawley rats were subjected to unilateral carotid artery ligation followed by 2.5h of hypoxia. Animals were randomly assigned to five groups: Sham (n = 8), Vehicle (n = 8), HI with G-CSF treatment (n = 9), HI with SCF treatment (n = 9), and HI with G-CSF + SCF treatment (coadministration group; n = 10). G-CSF (50μg/kg), SCF (50μg/kg), and G-CSF + SCF (50μg/kg) were administered intraperitoneally 1h post HI followed by daily injection for 4 consecutive days (five total injections). Animals were euthanized 14days after HI for neurological testing. Additionally, assessment of brain, heart, liver, spleen, and kidney atrophy was performed. Both G-CSF and G-CSF + SCF treatments improved body growth and decreased brain atrophy at 14days post HI. No significant differences were found in the peripheral organ weights between groups. Finally, the G-CSF + SCF coadministration group showed significant improvement in neurological function. Our data suggest that administration of G-CSF in combination with SCF not only prevented brain atrophy but also significantly improved neurological function. Springer Science+Business Media New York, 2012 Granulocyte-colony stimulating factor (G-CSF) nationallicence Stem cell factor (SCF) nationallicence Hypoxia-ischemia (HI) nationallicence Neurological outcome nationallicence Doycheva Desislava Department of Physiology & Pharmacology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut Shih Gary Department of Anaesthesiology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut Chen Hank Department of Physiology & Pharmacology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut Applegate Richard Department of Anaesthesiology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut Zhang John Department of Physiology & Pharmacology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut Tang Jiping Department of Physiology & Pharmacology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut Translational Stroke Research Springer-Verlag 4/2(2013-04-01), 171-178 1868-4483 4:2<171 2013 4 12975 https://doi.org/10.1007/s12975-012-0225-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12975-012-0225-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Doycheva Desislava Department of Physiology & Pharmacology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Shih Gary Department of Anaesthesiology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Chen Hank Department of Physiology & Pharmacology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Applegate Richard Department of Anaesthesiology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Zhang John Department of Physiology & Pharmacology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Tang Jiping Department of Physiology & Pharmacology, Loma Linda University School of Medicine, 92354, Loma Linda, CA, USA aut NATIONALLICENCE 773 0- Translational Stroke Research Springer-Verlag 4/2(2013-04-01), 171-178 1868-4483 4:2<171 2013 4 12975 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer