naa a22 4500 510786340 CHVBK 20180411083303.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12603-013-0353-8 doi (NATIONALLICENCE)springer-10.1007/s12603-013-0353-8 Effect of intermittent glutamine supplementation on skeletal muscle is not long-lasting in very old rats [Elektronische Daten] [Dominique Meynial-Denis, A. Beaufrere, M. Mignon, P. Patureau Mirand] Background and objective: Muscle is the major site for glutamine synthesis via glutamine synthetase (GS). This enzyme is increased 1.5-2 fold in 25-27-mo rats and may be a consequence of aging-induced stress. This stimulation is similar to the induction observed following a catabolic state such as glucocorticoid treatment (6 to 24 months). Although oral glutamine supply regulates the plasma glutamine level, nothing is known if this supplementation is interrupted before the experiment. Design: Adult (8-mo) and very old (27-mo) female rats were exposed to intermittent glutamine supplementation for 50 % of their age lifetime. Treated rats received glutamine added to their drinking water and control rats water alone but the effect of glutamine supplementation was only studied 15 days after the last supplementation. Results: Glutamine pretreatment discontinued 15 days before the experiment increased plasma glutamine to ∼ 0.6 mM, a normal value in very old rats. However, it failed to decrease the up-regulated GS activity in skeletal muscle from very old rats. Conclusion: Our results suggest that long-term treatment with glutamine started before advanced age but discontinued 15 days before rat sacrifice is effective in increasing plasma glutamine to recover basal adult value and in maintaining plasma glutamine in very old rats, but has no long-lasting effect on the GS activity of skeletal muscle with advanced age. Serdi and Springer-Verlag France, 2013 Glutamine synthetase activity nationallicence discontinuation nationallicence tibialis anterior muscle nationallicence glutamine level nationallicence plasma nationallicence advanced age nationallicence Meynial-Denis Dominique INRA, UMR 1019, UNH, CRNH Auvergne, 63009, Clermont-Ferrand, France aut Beaufrere A. CHU Clermont-Ferrand, Service d'Anatomie Pathologique, Hôpital Estaing, 63003, Clermont-Ferrand, Cedex1, France aut Mignon M. INRA, UMR 1019, UNH, CRNH Auvergne, 63009, Clermont-Ferrand, France aut Patureau Mirand P. INRA, UMR 1019, UNH, CRNH Auvergne, 63009, Clermont-Ferrand, France aut The journal of nutrition, health & aging Springer Paris 17/10(2013-12-01), 876-879 1279-7707 17:10<876 2013 17 12603 https://doi.org/10.1007/s12603-013-0353-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12603-013-0353-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meynial-Denis Dominique INRA, UMR 1019, UNH, CRNH Auvergne, 63009, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Beaufrere A. CHU Clermont-Ferrand, Service d'Anatomie Pathologique, Hôpital Estaing, 63003, Clermont-Ferrand, Cedex1, France aut NATIONALLICENCE 700 1- Mignon M. INRA, UMR 1019, UNH, CRNH Auvergne, 63009, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Patureau Mirand P. INRA, UMR 1019, UNH, CRNH Auvergne, 63009, Clermont-Ferrand, France aut NATIONALLICENCE 773 0- The journal of nutrition, health & aging Springer Paris 17/10(2013-12-01), 876-879 1279-7707 17:10<876 2013 17 12603 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer