naa a22 4500 510787274 CHVBK 20180411083306.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0300-9 doi (NATIONALLICENCE)springer-10.1007/s12041-013-0300-9 Cytosine hypomethylation at CHG and CHH sites in the pleiotropic mutants of Mendelian inheritance in Catharanthus roseus [Elektronische Daten] [RENU KUMARI, GITANJALI YADAV, VISHAKHA SHARMA, VINAY SHARMA, SUSHIL KUMAR] The 5S and 18S rDNA sequences of Catharanthus roseus cv ‘Nirmal' (wild type) and its leafless inflorescence (lli), evergreen dwarf (egd) and irregular leaf lamina (ill) single mutants and lli egd, lli ill and egd ill double mutants were characterized. The lli, egd and ill mutants of Mendelian inheritance bore the names after their most conspicuous morphological feature(s). They had been chemically induced and isolated for their salt tolerance. The double mutants were isolated as morphological segregants from crosses between single mutants. The morphological features of the two parents accompanied salt tolerance in the double mutants. All the six mutants were hypomethylated at repeat sequences, upregulated and downregulated for many genes and carried pleiotropic alterations for several traits. Here the 5S and 18S rDNAs of C.roseus were found to be relatively low in cytosine content. Cytosines were preponderantly in CG context (53%) and almost all of them were methylated (97%). The cytosines in CHH and CHG (where H = A, T or C) contexts were largely demethylated (92%) in mutants. The demethylation was attributable to reduced expression of RDR2 and DRM2 led RNA dependant DNA methylation and CMT3 led maintenance methylation pathways. Mutants had gained some cytosines by substitution of C at T sites. These perhaps arose on account of errors in DNA replication, mediated by widespread cytosine demethylation at CHG and CHH sites. It was concluded that the regulation of cytosine methylation mechanisms was disturbed in the mutants. ILL, EGD and LLI genes were identified as the positive regulators of other genes mediating the RdDM and CMT3 pathways, for establishment and maintenance of cytosine methylation in C.roseus. Indian Academy of Sciences, 2013 bisulphite sequencing nationallicence CMT3 nationallicence DNA demethylases nationallicence DNA methyl transferases nationallicence demethylation nationallicence excision repair nationallicence mutagenic hot spots nationallicence phylogenetic relationships nationallicence 5S rDNA nationallicence 18S rDNA nationallicence RdDM nationallicence spontaneous mutagenesis nationallicence KUMARI RENU Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut YADAV GITANJALI Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut SHARMA VISHAKHA Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut SHARMA VINAY Banasthali University, P.O. Banasthali Vidhyapeeth, 304 022, Banasthali, India aut KUMAR SUSHIL Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut Journal of Genetics Springer India 92/3(2013-12-01), 499-511 0022-1333 92:3<499 2013 92 12041 https://doi.org/10.1007/s12041-013-0300-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0300-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- KUMARI RENU Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut NATIONALLICENCE 700 1- YADAV GITANJALI Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut NATIONALLICENCE 700 1- SHARMA VISHAKHA Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut NATIONALLICENCE 700 1- SHARMA VINAY Banasthali University, P.O. Banasthali Vidhyapeeth, 304 022, Banasthali, India aut NATIONALLICENCE 700 1- KUMAR SUSHIL Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 499-511 0022-1333 92:3<499 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer