naa a22 4500 510787304 CHVBK 20180411083307.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0292-5 doi (NATIONALLICENCE)springer-10.1007/s12041-013-0292-5 Two P5CS genes from common bean exhibiting different tolerance to salt stress in transgenic Arabidopsis [Elektronische Daten] [JI CHEN, JIAN YANG, ZHAO ZHANG, XIAO FENG, SHU WANG] Many plants accumulate proline in response to salt stress. Δ-pyrroline-5-carboxylate synthetase (P5CS) is the rate-limiting enzyme in proline biosynthesis in plants. Plasmid DNA (pCHF3-PvP5CS1 and pCHF3-PvP5CS2) containing the selectable neomycin phosphotransferase gene for kanamycin resistance and Phaseolus vulgaris P5CS (PvP5CS1 and PvP5CS2) cDNA was introduced into Arabidopsis plants using Agrobacterium-mediated gene transfer. Southern blot, northern blot and RT-PCR analyses demonstrated that the foreign genes were integrated into Arabidopsis chromosomal DNA and expressed. Single-gene transformants were analysed in this study. Transgenic plants expressed higher levels of PvP5CS1 and PvP5CS2 transcripts under salt stress conditions than under normal conditions. When treated with 0, 100 and 200mM NaCl, the average proline content in leaves of transgenic plants was significantly higher (P < 0.01) than control plants. The average relative electrical conductivity (REC) of transgenic lines was significantly lower (P < 0.01) than control plants under salt stress condition. Biomass production of transgenic lines was significantly higher (P < 0.05) than control plants under 200mM NaCl stress treatment. These results indicated that introducing PvP5CS1 and PvP5CS2 cDNA into transgenic Arabidopsis caused proline overproduction, increasing salt tolerance. Although the expression of PvP5CS1 in L4 lines and PvP5CS2 in S4 lines was the same under salt stress condition, the S4 lines accumulated 1.6 and 1.9 times more proline than the L4 lines under 100 and 200mM NaCl treatments, respectively. The REC of S4 plants was 0.5 (100mM NaCl) and 0.6 times (200mM NaCl) that of L4 plants. The biomass production of S4 plants was 1.6 times (200mM NaCl) more than in L4 plants. Total P5CS enzyme activity of S4 was significantly higher than that of L4. These results implied that the PvP5CS2 protein had stronger capacity to catalyze proline synthesis than PvP5CS1 under salt stress condition. Indian Academy of Sciences, 2013 common bean nationallicence P5CS gene nationallicence salt resistance nationallicence proline nationallicence Arabidopsis nationallicence CHEN JI Nanyang Normal University, Wolong Road 1638, Wolong District, 473061, Nanyang City, People's Republic of China aut YANG JIAN Nanyang Normal University, Wolong Road 1638, Wolong District, 473061, Nanyang City, People's Republic of China aut ZHANG ZHAO Nanyang Normal University, Wolong Road 1638, Wolong District, 473061, Nanyang City, People's Republic of China aut FENG XIAO Nanyang Normal University, Wolong Road 1638, Wolong District, 473061, Nanyang City, People's Republic of China aut WANG SHU Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut Journal of Genetics Springer India 92/3(2013-12-01), 461-469 0022-1333 92:3<461 2013 92 12041 https://doi.org/10.1007/s12041-013-0292-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0292-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- CHEN JI Nanyang Normal University, Wolong Road 1638, Wolong District, 473061, Nanyang City, People's Republic of China aut NATIONALLICENCE 700 1- YANG JIAN Nanyang Normal University, Wolong Road 1638, Wolong District, 473061, Nanyang City, People's Republic of China aut NATIONALLICENCE 700 1- ZHANG ZHAO Nanyang Normal University, Wolong Road 1638, Wolong District, 473061, Nanyang City, People's Republic of China aut NATIONALLICENCE 700 1- FENG XIAO Nanyang Normal University, Wolong Road 1638, Wolong District, 473061, Nanyang City, People's Republic of China aut NATIONALLICENCE 700 1- WANG SHU Institute of Crop Sciences, The Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 461-469 0022-1333 92:3<461 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer