naa a22 4500 510787363 CHVBK 20180411083307.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0298-z doi (NATIONALLICENCE)springer-10.1007/s12041-013-0298-z Molecular characterization and expression analysis of fat mass and obesity-associated gene in rabbit [Elektronische Daten] [JINYI XING, WENQIAN JING, YUNLIANG JIANG] Fat mass and obesity-associated (FTO) gene codes for a nuclear protein of the AlkB related nonhaem iron and 2-oxoglutarate-dependent oxygenase superfamily, and is involved in animal fat deposition and human obesity. In this work, the molecular characterization and expression features of rabbit (Oryctolagus cuniculus) FTO cDNA were analysed. The rabbit FTO cDNA with a size of 2158bp was cloned, including 1515bp of the open reading frame that encoded a basic protein of 504 amino acids. Homologous comparison indicated that the rabbit FTO shared 36.36-91.88% identity with those from other species and phylogenetic analysis showed that the rabbit FTO is closely related to human, but more distantly related to zebrafish. The New Zealand rabbit FTO mRNA was detected in all tissues examined, with the highest levels found in the spleen and the lowest found in the kidney. However, no significant differences were seen in cerebellum, corpora quadrigemina, medulla oblongata and cerebral cortex of commercial adult rabbits. Moreover, mRNA levels of FTO in liver tissues were significantly increased in lactating New Zealand rabbits compared with 70-day-old, 90-day-old and gestating rabbits (P < 0.05). In contrast, FTO mRNA levels were significantly lower in longissimus dorsi muscle of 90-day-old New Zealand rabbits than in 70-day-old rabbits (P < 0.05). However, the expression levels of FTO in mammary gland and ovary of gestating and lactating rabbits were not significantly different (P > 0.05). Indian Academy of Sciences, 2013 fat mass and obesity-associated gene ( FTO ) nationallicence rabbit nationallicence mRNA expression patterns nationallicence sequence analysis nationallicence Oryctolagus cuniculus nationallicence XING JINYI College of Life Science, Linyi University, 276000, Linyi, People's Republic of China aut JING WENQIAN College of Life Science, Linyi University, 276000, Linyi, People's Republic of China aut JIANG YUNLIANG College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, Tai'an, People's Republic of China aut Journal of Genetics Springer India 92/3(2013-12-01), 481-488 0022-1333 92:3<481 2013 92 12041 https://doi.org/10.1007/s12041-013-0298-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0298-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- XING JINYI College of Life Science, Linyi University, 276000, Linyi, People's Republic of China aut NATIONALLICENCE 700 1- JING WENQIAN College of Life Science, Linyi University, 276000, Linyi, People's Republic of China aut NATIONALLICENCE 700 1- JIANG YUNLIANG College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, Tai'an, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 481-488 0022-1333 92:3<481 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer