naa a22 4500 510787401 CHVBK 20180411083307.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0297-0 doi (NATIONALLICENCE)springer-10.1007/s12041-013-0297-0 Cloning, characterization and expression of $\emph{OsFMO}_{{\mathbf{(}\emph{t}\mathbf{)}}}$ in rice encoding a flavin monooxygenase [Elektronische Daten] [JICAI YI, LANNA LIU, YOUPEI CAO, JIAZUO LI, MANTONG MEI] Flavin monooxygenases (FMO) play a key role in tryptophan (Trp)-dependent indole-acetic acid (IAA) biosynthesis in plants and regulate plant growth and development. In this study, the full-length genomic DNA and cDNA of OsFMO (t), a FMO gene that was originally identified from a rolled-leaf mutant in rice, was isolated and cloned from wild type of the rolled-leaf mutant. OsFMO (t) was found to have four exons and three introns, and encode a protein with 422 amino acid residues that contains two basic conserved motifs, with a ‘G×G××G' characteristic structure. OsFMO(t) showed high amino acid sequence identity with FMO proteins from other plants, in particular with YUCCA from Arabidopsis, FLOOZY from Petunia, and OsYUCCA1 from rice. Our phylogenetic analysis showed that OsFMO(t) and the homologous FMO proteins belong to the same clade in the evolutionary tree. Overexpression of OsFMO (t) in transformed rice calli produced IAA-excessive phenotypes that showed browning and lethal effects when exogenous auxins such as naphthylacetic acid (NAA) were added to the medium. These results suggested that the OsFMO(t) protein is involved in IAA biosynthesis in rice and its overexpression could lead to the malformation of calli. Spatio-temporal expression analysis using RT-PCR and histochemical analysis for GUS activity revealed that expression of OsFMO (t) was totally absent in the rolled-leaf mutant. However, in the wild type variety, this gene was expressed at different levels temporally and spatially, with the highest expression observed in tissues with fast growth and cell division such as shoot apexes, tender leaves and root tips. Our results demonstrated that IAA biosynthesis regulated by OsFMO (t) is likely localized and might play an essential role in shaping local IAA concentrations which, in turn, is critical for regulating normal growth and development in rice. Indian Academy of Sciences, 2013 cloning nationallicence characterization nationallicence OsFMO ( t ) gene nationallicence flavin monooxygenase nationallicence indole-acetic acid nationallicence expression analysis nationallicence YI JICAI Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Science, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut LIU LANNA College of Life Science, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut CAO YOUPEI Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Science, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut LI JIAZUO Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Science, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut MEI MANTONG Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Science, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Journal of Genetics Springer India 92/3(2013-12-01), 471-480 0022-1333 92:3<471 2013 92 12041 https://doi.org/10.1007/s12041-013-0297-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0297-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- YI JICAI Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Science, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- LIU LANNA College of Life Science, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- CAO YOUPEI Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Science, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- LI JIAZUO Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Science, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- MEI MANTONG Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Science, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 471-480 0022-1333 92:3<471 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer