naa a22 4500 510787436 CHVBK 20180411083307.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0306-3 doi (NATIONALLICENCE)springer-10.1007/s12041-013-0306-3 Identification and expression analysis of primary auxin-responsive Aux/IAA gene family in cucumber ( Cucumis sativus ) [Elektronische Daten] [DEFANG GAN, DAN ZHUANG, FEI DING, ZHENZHOU YU, YANG ZHAO] Aux/IAA is an important gene family involved in many aspects of growth and development. Aux/IAA proteins are short-lived nuclear proteins that are induced primarily by various phytohormones. In this study, 29 Aux/IAA family genes (CsIAA01-CsIAA29) were identified and characterized in cucumber, including gene structures, phylogenetic relationships, conserved protein motifs and chromosomal locations. These genes show distinct organizational patterns of their putative motifs. The distributions of the genes vary: except for five CsIAA genes in cucumber that were not located, seven CsIAA genes were found on scaffold, while the other 17 CsIAA genes were distributed on seven other chromosomes. Based on a phylogenetic analysis of the Aux/IAA protein sequences from cucumber, Arabidopsis and other plants, the Aux/IAA genes in cucumber were categorized into seven subfamilies. To investigate whether the expression of CsIAA genes is associated with auxin induction, their transcript levels were monitored in seedlings treated with IAA (indole-3-acetic acid), and their expression patterns were analysed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The results showed that 11/29 CsIAA genes were expressed in leaves whether treated with IAA or not and the time course of processing and compared with the control, five CsIAA genes showed low expression only after 60min treatment with IAA, while 11 genes showed no expression. These results provide useful information for further functional analysis of Aux/IAA gene family in cucumber. Indian Academy of Sciences, 2013 auxin nationallicence Aux/IAA nationallicence bioinformatic analysis nationallicence cucumber nationallicence gene expression nationallicence GAN DEFANG Anhui Province Key Laboratory of Crop Biology, School of Life Science, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut ZHUANG DAN School of Horticulture, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut DING FEI School of Horticulture, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut YU ZHENZHOU School of Horticulture, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut ZHAO YANG Anhui Province Key Laboratory of Crop Biology, School of Life Science, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut Journal of Genetics Springer India 92/3(2013-12-01), 513-521 0022-1333 92:3<513 2013 92 12041 https://doi.org/10.1007/s12041-013-0306-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0306-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- GAN DEFANG Anhui Province Key Laboratory of Crop Biology, School of Life Science, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut NATIONALLICENCE 700 1- ZHUANG DAN School of Horticulture, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut NATIONALLICENCE 700 1- DING FEI School of Horticulture, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut NATIONALLICENCE 700 1- YU ZHENZHOU School of Horticulture, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut NATIONALLICENCE 700 1- ZHAO YANG Anhui Province Key Laboratory of Crop Biology, School of Life Science, Anhui Agricultural University, 230036, Hefei, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 513-521 0022-1333 92:3<513 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer