naa a22 4500 510787487 CHVBK 20180411083307.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0312-5 doi (NATIONALLICENCE)springer-10.1007/s12041-013-0312-5 Assessment of genetic diversity in Indian rice germplasm ( Oryza sativa L.): use of random versus trait-linked microsatellite markers [Elektronische Daten] [SHEEL YADAV, ASHUTOSH SINGH, M. SINGH, NITIKA GOEL, K. VINOD, T. MOHAPATRA, A. SINGH] Assessment of genetic diversity in a crop germplasm is a vital part of plant breeding. DNA markers such as microsatellite or simple sequence repeat markers have been widely used to estimate the genetic diversity in rice. The present study was carried out to decipher the pattern of genetic diversity in terms of both phenotypic and genotypic variability, and to assess the efficiency of random vis-à-vis QTL linked/gene based simple sequence repeat markers in diversity estimation. A set of 88 rice accessions that included landraces, farmer's varieties and popular Basmati lines were evaluated for agronomic traits and molecular diversity. The random set of SSR markers included 50 diversity panel markers developed under IRRI's Generation Challenge Programme (GCP) and the trait-linked/gene based markers comprised of 50 SSR markers reportedly linked to yield and related components. For agronomic traits, significant variability was observed, ranging between the maximum for grains/panicle and the minimum for panicle length. The molecular diversity based grouping indicated that varieties from a common centre were genetically similar, with few exceptions. The trait-linked markers gave an average genetic dissimilarity of 0.45 as against that of 0.37 by random markers, along with an average polymorphic information constant value of 0.48 and 0.41 respectively. The correlation between the kinship matrix generated by trait-linked markers and the phenotype based distance matrix (0.29) was higher than that of random markers (0.19). This establishes the robustness of trait-linked markers over random markers in estimating genetic diversity of rice germplasm. Indian Academy of Sciences, 2013 germplasm nationallicence genetic diversity nationallicence microsatellite markers nationallicence principal component analysis nationallicence YADAV SHEEL Division of Genetics, Indian Agricultural Research Institute, 110 012, New Delhi, India aut SINGH ASHUTOSH Division of Genetics, Indian Agricultural Research Institute, 110 012, New Delhi, India aut SINGH M. Post Graduate College, 233001, Ghazipur, India aut GOEL NITIKA Division of Genetics, Indian Agricultural Research Institute, 110 012, New Delhi, India aut VINOD K. Indian Agricultural Research Institute, Rice Breeding and Genetics Research Centre, 612 101, Aduthurai, India aut MOHAPATRA T. Central Rice Research Institute, 753 006, Cuttack, India aut SINGH A. Division of Genetics, Indian Agricultural Research Institute, 110 012, New Delhi, India aut Journal of Genetics Springer India 92/3(2013-12-01), 545-557 0022-1333 92:3<545 2013 92 12041 https://doi.org/10.1007/s12041-013-0312-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0312-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- YADAV SHEEL Division of Genetics, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- SINGH ASHUTOSH Division of Genetics, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- SINGH M. Post Graduate College, 233001, Ghazipur, India aut NATIONALLICENCE 700 1- GOEL NITIKA Division of Genetics, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- VINOD K. Indian Agricultural Research Institute, Rice Breeding and Genetics Research Centre, 612 101, Aduthurai, India aut NATIONALLICENCE 700 1- MOHAPATRA T. Central Rice Research Institute, 753 006, Cuttack, India aut NATIONALLICENCE 700 1- SINGH A. Division of Genetics, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 545-557 0022-1333 92:3<545 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer