naa a22 4500 510787495 CHVBK 20180411083307.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0299-y doi (NATIONALLICENCE)springer-10.1007/s12041-013-0299-y Analysis of regulatory networks constructed based on gene coexpression in pituitary adenoma [Elektronische Daten] [JIE GONG, BO DIAO, GUO YAO, YING LIU, GUO XU] Gene coexpression patterns can reveal gene collections with functional consistency. This study systematically constructs regulatory networks for pituitary tumours by integrating gene coexpression, transcriptional and posttranscriptional regulation. Through network analysis, we elaborate the incidence mechanism of pituitary adenoma. The Pearson's correlation coefficient was utilized to calculate the level of gene coexpression. By comparing pituitary adenoma samples with normal samples, pituitary adenoma-specific gene coexpression patterns were identified. For pituitary adenoma-specific coexpressed genes, we integrated transcription factor (TF) and microRNA (miRNA) regulation to construct a complex regulatory network from the transcriptional and posttranscriptional perspectives. Network module analysis identified the synergistic regulation of genes by miRNAs and TFs in pituitary adenoma. We identified 142 pituitary adenoma-specific active genes, including 43 TFs and 99 target genes of TFs. Functional enrichment of these 142 genes revealed that the occurrence of pituitary adenoma induced abnormalities in intracellular metabolism and angiogenesis process. These 142 genes were also significantly enriched in adenoma pathway. Module analysis of the systematic regulatory network found that three modules contained elements that were closely related to pituitary adenoma, such as FGF2 and SP1, as well as transcription factors and miRNAs involved in the tumourigenesis. These results show that in the occurrence of pituitary adenoma, miRNA, TF and genes interact with each other. Based on gene expression, the proposed method integrates interaction information from different levels and systematically explains the occurrence of pituitary tumours. It facilitates the tracing of the origin of the disease and can provide basis for early diagnosis of complex diseases or cancer without obvious symptoms. Indian Academy of Sciences, 2013 coexpression network nationallicence transcription factor nationallicence miRNAs nationallicence functional enrichment nationallicence GONG JIE Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut DIAO BO Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut YAO GUO Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut LIU YING Department of Laboratory Technology, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut XU GUO Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut Journal of Genetics Springer India 92/3(2013-12-01), 489-497 0022-1333 92:3<489 2013 92 12041 https://doi.org/10.1007/s12041-013-0299-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0299-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- GONG JIE Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut NATIONALLICENCE 700 1- DIAO BO Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut NATIONALLICENCE 700 1- YAO GUO Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut NATIONALLICENCE 700 1- LIU YING Department of Laboratory Technology, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut NATIONALLICENCE 700 1- XU GUO Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, 430070, Wuhan, Hubei Province, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 489-497 0022-1333 92:3<489 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer