naa a22 4500 510787525 CHVBK 20180411083307.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0305-4 doi (NATIONALLICENCE)springer-10.1007/s12041-013-0305-4 Molecular analysis of intragenic recombination at the tryptophan synthetase locus in Neurospora crassa [Elektronische Daten] [A. WIEST, D. BARCHERS, M. EATON, R. HENDERSON, R. SCHNITTKER, K. MCCLUSKEY] Fifteen different classically generated and mapped mutations at the tryptophan synthetase locus in Neurospora crassa have been characterized to the level of the primary sequence of the gene. This sequence analysis has demonstrated that intragenic recombination is accurate to order mutations within one open reading frame. While classic genetic analysis correctly ordered the mutations, the position of mutations characterized by gene sequence analysis was more accurate. A leaky mutation was found to have a wild-type primary sequence. The presence of unique polymorphisms in the primary sequence of the trp-3 gene from strain 861 confirms that it has a unique history relative to the other strains studied. Most strains that were previously shown to be immunologically nonreactive with antibody preparations raised against tryptophan synthetase protein were shown to have nonsense mutations. This work defines 14 alleles of the N. crassa trp-3 gene. Indian Academy of Sciences, 2013 mutation nationallicence recombination nationallicence DNA sequence nationallicence primary metabolism nationallicence trp-3 nationallicence WIEST A. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut BARCHERS D. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut EATON M. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut HENDERSON R. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut SCHNITTKER R. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut MCCLUSKEY K. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut Journal of Genetics Springer India 92/3(2013-12-01), 523-528 0022-1333 92:3<523 2013 92 12041 https://doi.org/10.1007/s12041-013-0305-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0305-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- WIEST A. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut NATIONALLICENCE 700 1- BARCHERS D. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut NATIONALLICENCE 700 1- EATON M. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut NATIONALLICENCE 700 1- HENDERSON R. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut NATIONALLICENCE 700 1- SCHNITTKER R. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut NATIONALLICENCE 700 1- MCCLUSKEY K. Fungal Genetics Stock Center, University of Missouri, 64110, Kansas City, MO, USA aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 523-528 0022-1333 92:3<523 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer