naa a22 4500 51078755X CHVBK 20180411083307.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0275-6 doi (NATIONALLICENCE)springer-10.1007/s12041-013-0275-6 Expression profile of genes coding for carotenoid biosynthetic pathway during ripening and their association with accumulation of lycopene in tomato fruits [Elektronische Daten] [SHUCHI SMITA, RAVI RAJWANSHI, SANGRAM LENKA, AMIT KATIYAR, VISWANATHAN CHINNUSAMY, KAILASH BANSAL] Fruit ripening process is associated with change in carotenoid profile and accumulation of lycopene in tomato (Solanum lycopersicum L.). In this study, we quantified the β-carotene and lycopene content at green, breaker and red-ripe stages of fruit ripening in eight tomato genotypes by using high-performance liquid chromatography. Among the genotypes, lycopene content was found highest in Pusa Rohini and lowest in VRT-32-1. To gain further insight into the regulation of lycopene biosynthesis and accumulation during fruit ripening, expression analysis of nine carotenoid pathway-related genes was carried out in the fruits of high lycopene genotype—Pusa Rohini. We found that expression of phytoene synthase and β-carotene hydroxylase-1 was four and thirty-fold higher, respectively, at breaker stage as compared to red-ripe stage of fruit ripening. Changes in the expression level of these genes were associated with a 40% increase in lycopene content at red-ripe stage as compared with breaker stage. Thus, the results from our study suggest the role of specific carotenoid pathway-related genes in accumulation of high lycopene during the fruit ripening processes. Indian Academy of Sciences, 2013 carotenoid biosynthesis nationallicence carotenoid pathway genes nationallicence tomato nationallicence lycopene nationallicence Pusa Rohini nationallicence ripening nationallicence SMITA SHUCHI National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut RAJWANSHI RAVI National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut LENKA SANGRAM National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut KATIYAR AMIT National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut CHINNUSAMY VISWANATHAN Division of Plant Physiology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut BANSAL KAILASH National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut Journal of Genetics Springer India 92/3(2013-12-01), 363-368 0022-1333 92:3<363 2013 92 12041 https://doi.org/10.1007/s12041-013-0275-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0275-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- SMITA SHUCHI National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- RAJWANSHI RAVI National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- LENKA SANGRAM National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- KATIYAR AMIT National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- CHINNUSAMY VISWANATHAN Division of Plant Physiology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- BANSAL KAILASH National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, 110 012, New Delhi, India aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 363-368 0022-1333 92:3<363 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer