naa a22 4500 510787614 CHVBK 20180411083308.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s12041-013-0271-x doi (NATIONALLICENCE)springer-10.1007/s12041-013-0271-x Pleiotropic phenotypes of the salt-tolerant and cytosine hypomethylated leafless inflorescence , evergreen dwarf and irregular leaf lamina mutants of Catharanthus roseus possessing Mendelian inheritance [Elektronische Daten] [RENU KUMARI, VISHAKHA SHARMA, VINAY SHARMA, SUSHIL KUMAR] In Catharanthus roseus, three morphological cum salt-tolerant chemically induced mutants of Mendelian inheritance and their wild-type parent cv Nirmal were characterized for overall cytosine methylation at DNA repeats, expression of 119 protein-coding and seven miRNA-coding genes and 50 quantitative traits. The mutants, named after their principal morphological feature(s), were leafless inflorescence (lli), evergreen dwarf (egd) and irregular leaf lamina (ill). The Southern-blot analysis of MspI digested DNAs of mutants probed with centromeric and 5S and 18S rDNA probes indicated that, in comparison to wild type, the mutants were extensively demethylated at cytosine sites. Among the 126 genes investigated for transcriptional expression, 85 were upregulated and 41 were downregulated in mutants. All of the five genes known to be stress responsive had increased expression in mutants. Several miRNA genes showed either increased or decreased expression in mutants. The C. roseus counterparts of CMT3, DRM2 and RDR2 were downregulated in mutants. Among the cell, organ and plant size, photosynthesis and metabolism related traits studied, 28 traits were similarly affected in mutants as compared to wild type. Each of the mutants also expressed some traits distinctively. The egd mutant possessed superior photosynthesis and water retention abilities. Biomass was hyperaccumulated in roots, stems, leaves and seeds of the lli mutant. The ill mutant was richest in the pharmaceutical alkaloids catharanthine, vindoline, vincristine and vinblastine. The nature of mutations, origins of mutant phenotypes and evolutionary importance of these mutants are discussed. Indian Academy of Sciences, 2013 centromeric DNA nationallicence DNA hypomethylation nationallicence drought tolerance nationallicence epigenetic regulation nationallicence gene expression changes nationallicence microRNA nationallicence plant development nationallicence ribosomal DNA nationallicence salinity tolerance nationallicence terpenoid indole alkaloids nationallicence KUMARI RENU National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut SHARMA VISHAKHA National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut SHARMA VINAY Banasthali University, P. O. Banasthali Vidhyapeeth, 304 022, Banasthali, India aut KUMAR SUSHIL National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut Journal of Genetics Springer India 92/3(2013-12-01), 369-394 0022-1333 92:3<369 2013 92 12041 https://doi.org/10.1007/s12041-013-0271-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0271-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- KUMARI RENU National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut NATIONALLICENCE 700 1- SHARMA VISHAKHA National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut NATIONALLICENCE 700 1- SHARMA VINAY Banasthali University, P. O. Banasthali Vidhyapeeth, 304 022, Banasthali, India aut NATIONALLICENCE 700 1- KUMAR SUSHIL National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, 110 067, New Delhi, India aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/3(2013-12-01), 369-394 0022-1333 92:3<369 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer