naa a22 4500 51078819X CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s12041-013-0261-z doi (NATIONALLICENCE)springer-10.1007/s12041-013-0261-z Distribution pattern of histone H3 phosphorylation at serine 10 during mitosis and meiosis in Brachiaria species [Elektronische Daten] [C. PAULA, V. TECHIO, F. SOBRINHO, A. FREITAS] Histones are the major eukaryotic DNA-binding proteins. Posttranslational modifications on N-terminal tails of histones that form nucleosomes are often associated with distinct biological functions. Some theories suggest that one of these modifications, the phosphorylation of histone H3 at serine 10 (H3S10ph) plays a role in both chromosome condensation and sister chromatid cohesion. Although histones and some of their modifications are highly conserved, studies have shown that role and distribution of H3S10ph may differ between species. We evaluated the pattern of H3 phosphorylation using immunodetection during mitosis and meiosis in both diploid and tetraploid genotypes of Brachiaria species. Results revealed differences in chromosome distribution of H3S10ph when mitosis and meiosis were compared. Whole chromosomes were phosphorylated during meiosis I, whereas phosphorylation was restricted to the pericentromeric region in both meiosis II and mitosis. There was no variation in phosphorylation patterns between Brachiaria species and diploid and tetraploid genotypes. Regarding spatiotemporal coordination in the Brachiaria species evaluated, H3S10ph is related to maintenance of sister chromatid cohesion during cell divisions. Indian Academy of Sciences, 2013 sister chromatid cohesion nationallicence cell division nationallicence epigenetics nationallicence histone phosphorylation nationallicence immunodetection nationallicence PAULA C. Genetics and Plant Breeding, Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP: 372000-000, Lavras-MG, Brazil aut TECHIO V. Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP: 372000-000, Lavras-MG, Brazil aut SOBRINHO F. Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Embrapa Gado de Leite (CNPGL), Rua Eugênio do Nascimento, 610, Bairro Dom Bosco, CEP: 36038-330, Juiz de Fora-MG, Brazil aut FREITAS A. Biological Sciences, Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP: 372000-000, Lavras-MG, Brazil aut Journal of Genetics Springer India 92/2(2013-08-01), 259-266 0022-1333 92:2<259 2013 92 12041 https://doi.org/10.1007/s12041-013-0261-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0261-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- PAULA C. Genetics and Plant Breeding, Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP: 372000-000, Lavras-MG, Brazil aut NATIONALLICENCE 700 1- TECHIO V. Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP: 372000-000, Lavras-MG, Brazil aut NATIONALLICENCE 700 1- SOBRINHO F. Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Embrapa Gado de Leite (CNPGL), Rua Eugênio do Nascimento, 610, Bairro Dom Bosco, CEP: 36038-330, Juiz de Fora-MG, Brazil aut NATIONALLICENCE 700 1- FREITAS A. Biological Sciences, Universidade Federal de Lavras (UFLA), Campus Universitário, Caixa Postal 3037, CEP: 372000-000, Lavras-MG, Brazil aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/2(2013-08-01), 259-266 0022-1333 92:2<259 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer