naa a22 4500 510788203 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s12041-013-0238-y doi (NATIONALLICENCE)springer-10.1007/s12041-013-0238-y Isolation, characterization and mapping of genes differentially expressed during fibre development between Gossypium hirsutum and G. barbadense by cDNA-SRAP [Elektronische Daten] [CHUANXIANG LIU, DAOJUN YUAN, XIANLONG ZHANG, ZHONGXU LIN] Gossypium hirsutum and G. barbadense are two cultivated tetraploid cotton species with differences in fibre quality. The fibre of G. barbadense is longer, stronger and finer than that of G. hirsutum. To isolate genes expressed differently between the two species during fibre development, cDNA-SRAP (sequence-related amplified polymorphism) was applied. This technique was used to analyse genes at different stages of fibre development in G. hirsutum cv. Emian22 and G. barbadense acc. 3-79, the parents of our interspecific mapping population. A total of 4096 SRAP primer combinations were used to screen polymorphism between the DNA of the parents, and 275 highly polymorphic primers were picked out to analyse DNA and RNA from leaves and fibres at different developmental stages of the parents. A total of 168 DNA fragments were isolated from gels and sequenced: 54, 30, 38 and 41 from fibres of 5, 10, 15 and 20days post-anthesis, respectively, and five from multi stages. To genetically map these sequences, 104 sequence-specific primers were developed and were used to screened polymorphism between the mapping parents. Finally, six markers were mapped on six chromosomes of our backbone interspecific genetic map. This work can give us a primary knowledge of differences in mechanism of fibre development between G. hirsutum and G. barbadense. Indian Academy of Sciences, 2013 cotton nationallicence fibre development nationallicence cDNA-SRAP nationallicence genetic mapping nationallicence LIU CHUANXIANG National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut YUAN DAOJUN National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut ZHANG XIANLONG National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut LIN ZHONGXU National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut Journal of Genetics Springer India 92/2(2013-08-01), 175-181 0022-1333 92:2<175 2013 92 12041 https://doi.org/10.1007/s12041-013-0238-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0238-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- LIU CHUANXIANG National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- YUAN DAOJUN National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- ZHANG XIANLONG National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- LIN ZHONGXU National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/2(2013-08-01), 175-181 0022-1333 92:2<175 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer