naa a22 4500 510788211 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s12041-013-0246-y doi (NATIONALLICENCE)springer-10.1007/s12041-013-0246-y Preliminary genetic linkage maps of Chinese herb Dendrobium nobile and D. moniliforme [Elektronische Daten] [SHANGGUO FENG, HONGYAN ZHAO, JIANGJIE LU, JUNJUN LIU, BO SHEN, HUIZHONG WANG] Dendrobium is an endangered genus in the orchid family with medicinal and horticultural value. Two preliminary genetic linkage maps were constructed using 90 F1 progeny individuals derived from an interspecific cross between D. nobile and D. moniliforme (both, 2n = 38), using random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR). A total of 286 RAPD loci and 68 ISSR loci were identified and used for genetic linkage analysis. Maps were constructed by double pseudo-testcross mapping strategy using the software Mapmaker/EXP ver. 3.0, and Kosambi map distances were constructed using a LOD score ≥ 4 and a recombination threshold of 0.4. The resulting frame map of D. nobile was 1474cM in total length with 116 loci distributed in 15 linkage groups; and the D. moniliforme linkage map had 117 loci placed in 16 linkage groups spanning 1326.5cM. Both maps showed 76.91% and 73.59% genome coverage for D. nobile and D. moniliforme, respectively. These primary maps provide an important basis for genetic studies and further medicinal and horticultural traits mapping and marker-assisted selection in Dendrobium breeding programmes. Indian Academy of Sciences, 2013 Dendrobium nationallicence double pseudo-testcross nationallicence genetic linkage nationallicence RAPD nationallicence ISSR nationallicence FENG SHANGGUO Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut ZHAO HONGYAN Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut LU JIANGJIE Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut LIU JUNJUN Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut SHEN BO Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut WANG HUIZHONG Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut Journal of Genetics Springer India 92/2(2013-08-01), 205-212 0022-1333 92:2<205 2013 92 12041 https://doi.org/10.1007/s12041-013-0246-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12041-013-0246-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- FENG SHANGGUO Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- ZHAO HONGYAN Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- LU JIANGJIE Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- LIU JUNJUN Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- SHEN BO Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- WANG HUIZHONG Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, 310018, Hangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Genetics Springer India 92/2(2013-08-01), 205-212 0022-1333 92:2<205 2013 92 12041 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer