naa a22 4500 51078822X CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12298-012-0148-0 doi (NATIONALLICENCE)springer-10.1007/s12298-012-0148-0 Micropropagation of seedless lemon ( Citrus limon L. cv. Kaghzi Kalan) and assessment of genetic fidelity of micropropagated plants using RAPD markers [Elektronische Daten] [Komal Goswami, R. Sharma, P. Singh, Govind Singh] A micropropagation protocol was developed for multiplication of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) using nodal explants. The maximum shoot regeneration was observed on low level of BAP (0.1mg l−1) or kinetin (0.5mg l−1). BA was recorded to be better than kinetin in terms of number of days taken to bud break. The maximum number of shoots per explant was observed on 0.1mg l−1 BA and 0.5mg l−1 kinetin. Shoot proliferation decreased with increasing concentration of BA alone, but in case of a combination of BA and NAA (0.1mg l−1 each), it increased with increasing concentration of BA up to 10.0mg l−1. None of the treatments including BA or kinetin alone or BA in combination with NAA produced significantly more shoots for commercial exploitation. In the case of a combination of BA + kinetin + IBA, the maximum (5.5 shoots per explants) proliferation was observed on MS medium containing 1.0mg l−1 BA + 0.5mg l−1 kinetin + 0.5mg l−1 IBA or 0.25mg l−1 BA + 1.0mg l−1 kinetin + 1.0mg l−1 IBA. Regenerated shoots showed root induction on MS basal medium or on MS medium containing 1.0mg l−1 IBA. It is concluded that a five-fold increase (1.0mg l−1 BA + 0.5mg l−1 kinetin + 0.5mg l−1 IBA) in axillary shoot proliferation, while seven-fold increase (0.25mg/l mg l−1 BA + 1.0mg l−1 kinetin + 1.0mg l−1 IBA) during the second cycle of multiplication could be obtained using the two plant growth regulator combinations. PCR amplification with 14 different random primers confirmed no somaclonal variant up to two cycles of shoot multiplication. Prof. H.S. Srivastava Foundation for Science and Society, 2012 Micropropagation nationallicence Regeneration nationallicence In vitro nationallicence Genetic fidelity nationallicence RAPD nationallicence Goswami Komal Plant Biotechnology Center, SKN Rajasthan Agricultural University, Beechwal, 334006, Bikaner, Rajasthan, India aut Sharma R. Plant Biotechnology Center, SKN Rajasthan Agricultural University, Beechwal, 334006, Bikaner, Rajasthan, India aut Singh P. Plant Biotechnology Center, SKN Rajasthan Agricultural University, Beechwal, 334006, Bikaner, Rajasthan, India aut Singh Govind Plant Biotechnology Center, SKN Rajasthan Agricultural University, Beechwal, 334006, Bikaner, Rajasthan, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 137-145 0971-5894 19:1<137 2013 19 12298 https://doi.org/10.1007/s12298-012-0148-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0148-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goswami Komal Plant Biotechnology Center, SKN Rajasthan Agricultural University, Beechwal, 334006, Bikaner, Rajasthan, India aut NATIONALLICENCE 700 1- Sharma R. Plant Biotechnology Center, SKN Rajasthan Agricultural University, Beechwal, 334006, Bikaner, Rajasthan, India aut NATIONALLICENCE 700 1- Singh P. Plant Biotechnology Center, SKN Rajasthan Agricultural University, Beechwal, 334006, Bikaner, Rajasthan, India aut NATIONALLICENCE 700 1- Singh Govind Plant Biotechnology Center, SKN Rajasthan Agricultural University, Beechwal, 334006, Bikaner, Rajasthan, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 137-145 0971-5894 19:1<137 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer