naa a22 4500 510788262 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12298-012-0150-6 doi (NATIONALLICENCE)springer-10.1007/s12298-012-0150-6 Micropropagation of annatto ( Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers [Elektronische Daten] [E. Siril, Nisha Joseph] An in vitro propagation technique based on axillary bud proliferation was developed for the first time to mature annatto (Bixa orellana L.) tree. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with 1.0μM benzyl adenine (BA) and tender coconut water (10%) showed significantly high (P < 0.05) explant response (67.0%), development of elongated shoots (3.36), shoot buds (8.9) and shoot elongation (3.53cm). Cytokinins like zeatin, isopentenyl adenine (2-iP), kinetin, or thidiazuron (TDZ) were inferior to BA to induce multiple shoots. Seasonal variations significantly affected the in vitro response of nodal explants. In vitro rooting experiments have showed 55.6% rooting on MS medium containing 15μM indole-3-butyric acid (IBA). Alternatively, in vitro raised shoots were rooted (61.1%) ex vitro, by 10mM indole-3-butyric acid (IBA) for 30s. The results of the RAPD marker system revealed the genetic stability among the micropropagated plants. The present protocol in brief, can be used for the clonal propagation of the superior genotype and preservation of germplasm. Prof. H.S. Srivastava Foundation for Science and Society, 2012 Benzyl adenine nationallicence Ex vitro rooting nationallicence Multiple shoots nationallicence Nodal segment nationallicence Tender coconut water nationallicence Siril E. Department of Botany, University of Kerala, Kariavattom, 695 581, Thiruvananthapuram, India aut Joseph Nisha Department of Botany, University of Kerala, Kariavattom, 695 581, Thiruvananthapuram, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 147-155 0971-5894 19:1<147 2013 19 12298 https://doi.org/10.1007/s12298-012-0150-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0150-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Siril E. Department of Botany, University of Kerala, Kariavattom, 695 581, Thiruvananthapuram, India aut NATIONALLICENCE 700 1- Joseph Nisha Department of Botany, University of Kerala, Kariavattom, 695 581, Thiruvananthapuram, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 147-155 0971-5894 19:1<147 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer