naa a22 4500 510788319 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12298-012-0144-4 doi (NATIONALLICENCE)springer-10.1007/s12298-012-0144-4 Antioxidative responses of Salvinia ( Salvinia natans Linn.) to aluminium stress and it's modulation by polyamine [Elektronische Daten] [C. Mandal, N. Ghosh, Saborni Maiti, K. Das, Sudha Gupta, N. Dey, M. Adak] Antioxidative stress response of free-floating aquatic fern (Salvinia natans Linn.) was studied under increasing toxic amount of aluminium (Al) and its modulation by exogenous application of polymaine. Increased levels of superoxide (O 2 − ) and hydrogen peroxide (H2O2) species from affected tissues suggested that plants were undergoing oxidative stress and it was concominant with increased accumulation of Al in a dose dependent manner. Application of polyamine like putrescine (Put) led to a decrease in oxidative stress as revealed by reduced level of O 2 − and H2O2. Al toxicity resulted into decreased biomass that was ameliorated by the application of Put. The changes observed in lipid peroxidation (MDA) and protein oxidation also indicated that plats are undergoing Al induced oxidative stress. In order to circumvent the oxidative stress resulting from Al toxicity, plants enzymatic and nonenzymatic antioxidant pathways were active. The ratio of both oxidized and reduced cellular glutathione exhibited significant variation in response to Al stress and was improved upon Put treatment. Peroxidase and glutathione were upregulated whereas catalse was downregulated under varying doses of Al. Isozyme profile of above enzymes also showed a trend with increasing amount of Al. The nuclear disintegration study using comet assay was indicative of Al induced oxidative stress. In the present study, we have explored the antioxidative response of aquatic fern Salvinia natans Linn in response to Al toxicity. The application of polyamine Put improved the overall antioxidative response and thus would make it a better candidate to be used as hyper accumulator of Al and other toxic metals. Prof. H.S. Srivastava Foundation for Science and Society, 2012 Aluminium toxicity nationallicence Reactive oxygen species nationallicence Polyamine nationallicence Antioxidative enzymes nationallicence Comet assay nationallicence Salvinia sp nationallicence H2O2 : Hydrogen peroxide nationallicence Put : Putrescine nationallicence MDA : Malondealdehide nationallicence ROS : Reactive oxygen species nationallicence PCD : Programmed cell death nationallicence SOD : Superoxide dismutase nationallicence POD : Peroxidase nationallicence O 2 − : Superoxide nationallicence OH− : Hydroxyl ion nationallicence KAl(SO4)2 : 12 H2O, Potassium aluminium sulphate nationallicence IMZ : Imidazole nationallicence NaN3 : Sodium azide nationallicence TCA : Tricholoroacetic acid nationallicence KI : Potassium iodide nationallicence PVP : Polyvenyl pyroliddone nationallicence EDTA : Ethylene diamine tetra acetic acid nationallicence DTT : Dithiotreitol nationallicence NAD(P)H : Nicotinamide adenine dinucleotide phosphate (reduced form) nationallicence DNPH : Dinitrophenyl hydragene nationallicence DTNB : 5, 5′-dithio-bis (2 nitrobenzoic acid) nationallicence GSH : Glutathione nationallicence GSSG : Oxidized glutathione nationallicence GPX : Guaiacol peroxidase nationallicence APX : Ascorbate peroxidase nationallicence CAT : Catalase nationallicence GR : Glutathione reductase nationallicence BSA : Bovine serum albumin nationallicence PMSF : Phenyl methane sulfonyl fluoride nationallicence DCPIP : Dichlorophenol indophenol nationallicence MTT : 3-(4,5-dimethyl -2 thiazolil)-2,5 diphenyl-H tetrazolium bromine nationallicence PBS : Phosphate saline buffer nationallicence LMP : Low melting agarose nationallicence EtBr : Ethydium broimide nationallicence PAs : Polyamines nationallicence Mandal C. Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut Ghosh N. Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut Maiti Saborni Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut Das K. Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut Gupta Sudha Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut Dey N. Centre for Biotechnology, Visva Bharati University, 731235, Santiniketan, West Bengal, India aut Adak M. Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 91-103 0971-5894 19:1<91 2013 19 12298 https://doi.org/10.1007/s12298-012-0144-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0144-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mandal C. Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut NATIONALLICENCE 700 1- Ghosh N. Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut NATIONALLICENCE 700 1- Maiti Saborni Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut NATIONALLICENCE 700 1- Das K. Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut NATIONALLICENCE 700 1- Gupta Sudha Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut NATIONALLICENCE 700 1- Dey N. Centre for Biotechnology, Visva Bharati University, 731235, Santiniketan, West Bengal, India aut NATIONALLICENCE 700 1- Adak M. Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, 741235, Kalyani, West Bengal, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 91-103 0971-5894 19:1<91 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer