naa a22 4500 510788343 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12298-012-0149-z doi (NATIONALLICENCE)springer-10.1007/s12298-012-0149-z Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR) [Elektronische Daten] [Wesam Al Khateeb, Eman Bahar, Jamil Lahham, Dana Schroeder, Emad Hussein] Moringa peregrina is an endangered species of Moringaceae. M. peregrina is a multipurpose tree with a wide variety of potential uses including its medicinal activity. In our study, a rapid and efficient micropropagation protocol for M. peregrina has been established. In vitro germinated seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different levels of either 6-benzyladenine (BA) or kinetin (Kin). The maximum shoot proliferation of 6.5 shoots per explant with 100% shoot proliferation rate was observed on MS medium supplemented with 1.0mg/l BA. On the other hand, MS medium supplemented with 1mg/l indole-3-butyric acid (IBA) resulted in the maximum number of roots. Micropropagated plants were successfully acclimatized. Genetic stability of micropropagated plants was assessed using Inter-Simple Sequence Repeat (ISSR). The amplification products were monomorphic in all in vitro grown plants. No polymorphism was detected indicating the genetic integrity of in vitro propagated plants. This micropropagation protocol could be useful for raising genetically uniform plants for plant propagation and commercial cultivation. Prof. H.S. Srivastava Foundation for Science and Society, 2012 Micropropagation nationallicence In vitro nationallicence ISSR nationallicence Endangered nationallicence Genetic stability nationallicence Moringa peregrina nationallicence Al Khateeb Wesam Department of Biological Sciences, Yarmouk University, Irbid, Jordan aut Bahar Eman Department of Biological Sciences, Yarmouk University, Irbid, Jordan aut Lahham Jamil Department of Biological Sciences, Yarmouk University, Irbid, Jordan aut Schroeder Dana Department of Biological Sciences, University of Manitoba, Winnipeg, Canada aut Hussein Emad Department of Biological Sciences, Yarmouk University, Irbid, Jordan aut Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 157-164 0971-5894 19:1<157 2013 19 12298 https://doi.org/10.1007/s12298-012-0149-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0149-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Al Khateeb Wesam Department of Biological Sciences, Yarmouk University, Irbid, Jordan aut NATIONALLICENCE 700 1- Bahar Eman Department of Biological Sciences, Yarmouk University, Irbid, Jordan aut NATIONALLICENCE 700 1- Lahham Jamil Department of Biological Sciences, Yarmouk University, Irbid, Jordan aut NATIONALLICENCE 700 1- Schroeder Dana Department of Biological Sciences, University of Manitoba, Winnipeg, Canada aut NATIONALLICENCE 700 1- Hussein Emad Department of Biological Sciences, Yarmouk University, Irbid, Jordan aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 157-164 0971-5894 19:1<157 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer